BRENDA - Enzyme Database show
show all sequences of 2.7.1.149

The activity, evolution and association of phosphatidylinositol 5-phosphate 4-kinases

Clarke, J.; Irvine, R.; Adv. Biol. Regul. 52, 40-45 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
PI5P4Kalpha is predominantly in the cytoplasm, principally cytosolic, when recombinantly expressed
Mammalia
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
recombinantly expressed PI5P4Kalpha
Mammalia
5829
-
membrane
PI5P4Kalpha is membrane-bound in DT40 cells with a significant amount of endogenous PI5P4Kalpha in the nucleus
Mammalia
16020
-
additional information
analysis of subcellular localization of native and recombinant isozyme PI5P4Kalpha, overview
Mammalia
-
-
nucleus
PI5P4Kalpha is membrane-bound in DT40 cells with a significant amount of endogenous PI5P4Kalpha in the nucleus
Mammalia
5634
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Mammalia
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate
Mammalia
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mammalia
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
DT-40 cell
-
Mammalia
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate
-
721247
Mammalia
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Mammalia
Cloned(Commentary) (protein specific)
Commentary
Organism
PI5P4Kalpha is predominantly in the cytoplasm, principally cytosolic, when recombinantly expressed
Mammalia
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Mammalia
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
recombinantly expressed PI5P4Kalpha
Mammalia
5829
-
membrane
PI5P4Kalpha is membrane-bound in DT40 cells with a significant amount of endogenous PI5P4Kalpha in the nucleus
Mammalia
16020
-
additional information
analysis of subcellular localization of native and recombinant isozyme PI5P4Kalpha, overview
Mammalia
-
-
nucleus
PI5P4Kalpha is membrane-bound in DT40 cells with a significant amount of endogenous PI5P4Kalpha in the nucleus
Mammalia
5634
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Mammalia
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate
Mammalia
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
DT-40 cell
-
Mammalia
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate
-
721247
Mammalia
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
PI5P4Kalpha is present in all vertebrates, and a few organisms are missing either beta or gamma. Several key structural differences between the three mammalian PI5P4Ks largely account for their different activities, e.g. a glycine-rich loop supposed to be involved with substrate interaction
Mammalia
General Information (protein specific)
General Information
Commentary
Organism
evolution
PI5P4Kalpha is present in all vertebrates, and a few organisms are missing either beta or gamma. Several key structural differences between the three mammalian PI5P4Ks largely account for their different activities, e.g. a glycine-rich loop supposed to be involved with substrate interaction
Mammalia
Other publictions for EC 2.7.1.149
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739399
Tang
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Arabidopsis thaliana
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2016
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737401
Bulley
Exploring phosphatidylinositol ...
Homo sapiens
Adv. Biol. Regul.
57
193-202
2015
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737648
Clarke
The function of phosphatidylin ...
Mus musculus
Biochem. J.
466
359-367
2015
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1
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1
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738171
Boura
Phosphatidylinositol 4-kinases ...
Homo sapiens
Exp. Cell Res.
337
136-145
2015
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737598
Voss
Discovery and pharmacological ...
Homo sapiens
Biochem. Biophys. Res. Commun.
449
327-331
2014
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1
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20
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1
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1
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738639
Xu
Phosphoinositide kinases play ...
Rattus norvegicus
J. Biol. Chem.
289
6941-6948
2014
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739052
Bojjireddy
Type II phosphatidylinositol 4 ...
Homo sapiens
Mol. Cell. Biochem.
390
197-203
2014
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1
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1
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1
1
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723602
Davis
A homogeneous, high-throughput ...
Homo sapiens
PLoS ONE
8
e54127
2013
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15
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1
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1
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1
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1
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1
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1
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14
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15
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737638
Clarke
Evolutionarily conserved struc ...
Homo sapiens
Biochem. J.
454
49-57
2013
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1
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3
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7
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3
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3
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737789
Sinha
Type II phosphatidylinositol 4 ...
Homo sapiens
Biochimie
95
1560-1566
2013
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1
1
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738389
Elouarrat
Role of phosphatidylinositol 5 ...
Danio rerio
Int. J. Biochem. Cell Biol.
45
1293-1301
2013
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7
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7
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1
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1
1
1
1
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739535
Gupta
Phosphatidylinositol 5-phospha ...
Drosophila melanogaster
Proc. Natl. Acad. Sci. USA
110
5963-5968
2013
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2
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721247
Clarke
The activity, evolution and as ...
Mammalia
Adv. Biol. Regul.
52
40-45
2012
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1
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4
1
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1
1
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721493
Kouchi
Phosphatidylinositol 5-phospha ...
Homo sapiens
Biochem. Biophys. Res. Commun.
408
523-529
2011
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1
1
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1
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3
1
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2
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723270
Huang
Light-induced tyrosine phospho ...
Mus musculus, Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Neurochem. Res.
36
627-635
2011
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1
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5
2
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3
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166
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4
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721525
Bultsma
PIP4Kbeta interacts with and m ...
Homo sapiens, Rattus norvegicus
Biochem. J.
430
223-235
2010
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2
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2
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684375
Clarke
Localization of Phosphatidylin ...
Homo sapiens, Mus musculus
Am. J. Physiol. Renal Physiol.
295
F1422-1430
2008
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686033
Richardson
Genomic tagging of endogenous ...
Gallus gallus, Homo sapiens
Cell. Signal.
19
1309-1314
2007
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673010
Hinchliffe
Regulation of type II PIP kina ...
Sus scrofa
Cell. Signal.
18
1906-1913
2006
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663208
Carricaburu
The phosphatidylinositol (PI)- ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
100
9867-9872
2003
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393080
Itoh
A novel phosphatidylinositol-5 ...
Rattus norvegicus
J. Biol. Chem.
273
20292-20299
1998
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393079
Rameh
A new pathway for synthesis of ...
Homo sapiens
Nature
390
192-196
1997
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