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Literature summary for 2.7.1.148 extracted from

  • Liu, Z.; Jin, Y.; Tao, Y.; Wang, G.
    Crystal structure of IspF from Bacillus subtilis and absence of protein complex assembly amongst IspD/IspE/IspF enzymes in the MEP pathway (2018), Biosci. Rep., 38, BSR20171370 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.148

Cloned(Commentary)

Cloned (Comment) Organism
gene ispE, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) from pGEX-6P-1 expression vector Escherichia coli
gene ispE, recombinant expression of GST-tagged enzyme in Escherichia coli strain DH5alpha from pGEX-6P-1 expression vector Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli
Mg2+ required Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol Escherichia coli
-
 ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol Bacillus subtilis
-
 ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol Bacillus subtilis 168
-
 ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol Bacillus subtilis DSM 23778
-
 ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P37550
-
-
Bacillus subtilis 168 P37550
-
-
Bacillus subtilis DSM 23778 P37550
-
-
Escherichia coli P62615
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli strain DH5alpha by glutathione affinity chromatography, tag cleavage through Prescission protease, followed by anion exchange chromatography, and gel filtration Bacillus subtilis
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage through thrombin, followed by anion exchange chromatography, and gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 Escherichia coli ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 Bacillus subtilis ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 Bacillus subtilis 168 ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 Bacillus subtilis DSM 23778 ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Synonyms

Synonyms Comment Organism
BsIspE
-
 Bacillus subtilis
CDPME kinase
-
 Escherichia coli
CDPME kinase
-
 Bacillus subtilis
EcIspE
-
 Escherichia coli
IspE
-
 Escherichia coli
IspE
-
 Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli
ATP
-
 Bacillus subtilis

General Information

General Information Comment Organism
metabolism CDPME kinase (IspE) catalyzes the phosphorylation of CDPME to 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDPME2P) as part of the MEP pathway, overview. In Bacillus subtilis, no stable complexes of BsIspD/BsIspE, BsIspD/BsIspF, and BsIspD/BsIspE/BsIspF are observed, interaction analysis, overview Bacillus subtilis
metabolism CDPME kinase (IspE) catalyzes the phosphorylation of CDPME to 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDPME2P) as part of the MEP pathway, overview. In Escherichia coli, no stable complexes of EcIspD/EcIspE, EcIspE/EcIspF, and EcIspD/EcIspE/EcIspF are observed, only the dual mixture of BsIspE and BsIspF seems to merge into a single band, interaction analysis, overview Escherichia coli
physiological function CDPME kinase (IspE) catalyzes the phosphorylation of CDPME to 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDPME2P) Escherichia coli
physiological function CDPME kinase (IspE) catalyzes the phosphorylation of CDPME to 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDPME2P) Bacillus subtilis