BRENDA - Enzyme Database show
show all sequences of 2.7.1.148

Two copies of 4-(cytidine 5-diphospho)-2-C-methyl-D: -erythritol kinase (CMK) gene in Ginkgo biloba: molecular cloning and functional characterization

Kim, S.M.; Kim, Y.B.; Kuzuyama, T.; Kim, S.U.; Planta 228, 941-950 (2008)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
light
Selective increase in the transcript abundance of GbCMK2 in the radicles by induction of light.
Ginkgo biloba
methyl jasmonate
Selective increase in the transcript abundance of GbCMK2 in adicles.
Ginkgo biloba
Cloned(Commentary)
Commentary
Organism
The two CMK sequences encoding the mature CMK proteins are amplified with their respective primer pairs and cloned to the pMW118 vector to derive the pMW-CMK1 and the pMW-CMK2 plasmids. NMW29, an Escherichia coli ychB mutant harboring the pTMV20KM plasmid. The strain is transformed with the pMW-CMK1 and the pMW-CMK2 plasmids and selected on the LB medium without mevalonate.; The two CMK sequences encoding the mature CMK proteins are amplified with their respective primer pairs and cloned to the pMW118 vector to derive the pMW-CMK1 and the pMW-CMK2 plasmids. NMW29, an Escherichia coli ychB mutant harboring the pTMV20KM plasmid. The strain is transformed with the pMW-CMK1 and the pMW-CMK2 plasmids and selected on the LB medium without mevalonate.
Ginkgo biloba
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
isoenzyme contains chloroplast transit peptide sequence; isoenzyme contains chloroplast transit peptide sequence
Ginkgo biloba
9507
-
cytosol
-
Ginkgo biloba
5829
-
nucleus
-
Ginkgo biloba
5634
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39200
-
theoretical value, predicted
Ginkgo biloba
39900
-
theoretical value, predicted
Ginkgo biloba
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ATP
Ginkgo biloba
-
2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ADP
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ginkgo biloba
A6XAA8
-
-
Ginkgo biloba
A6XAA9
-
-
Reaction
Reaction
Commentary
Organism
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
catalyzes the fourth step of 2-C-methyl-D-erythritol 4-phosphate pathway; catalyzes the fourth step of 2-C-methyl-D-erythritol 4-phosphate pathway
Ginkgo biloba
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cotyledon
5 embryos are pooled to prepare a RNA sample, used for complementation assay; 5 embryos are pooled to prepare a RNA sample, used for complementation assay
Ginkgo biloba
-
radicle
5 embryos are pooled to prepare a RNA sample, used for complementation assay; 5 embryos are pooled to prepare a RNA sample, used for complementation assay
Ginkgo biloba
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ATP
-
689692
Ginkgo biloba
2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ADP
-
-
-
ir
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
;
Ginkgo biloba
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Ginkgo biloba
theoretical value, predicted
-
5.1
Ginkgo biloba
theoretical value, predicted
-
5.2
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
light
Selective increase in the transcript abundance of GbCMK2 in the radicles by induction of light.
Ginkgo biloba
methyl jasmonate
Selective increase in the transcript abundance of GbCMK2 in adicles.
Ginkgo biloba
Cloned(Commentary) (protein specific)
Commentary
Organism
The two CMK sequences encoding the mature CMK proteins are amplified with their respective primer pairs and cloned to the pMW118 vector to derive the pMW-CMK1 and the pMW-CMK2 plasmids. NMW29, an Escherichia coli ychB mutant harboring the pTMV20KM plasmid. The strain is transformed with the pMW-CMK1 and the pMW-CMK2 plasmids and selected on the LB medium without mevalonate.
Ginkgo biloba
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Ginkgo biloba
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
isoenzyme contains chloroplast transit peptide sequence
Ginkgo biloba
9507
-
cytosol
-
Ginkgo biloba
5829
-
nucleus
-
Ginkgo biloba
5634
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39200
-
theoretical value, predicted
Ginkgo biloba
39900
-
theoretical value, predicted
Ginkgo biloba
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ATP
Ginkgo biloba
-
2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ADP
-
-
ir
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cotyledon
5 embryos are pooled to prepare a RNA sample, used for complementation assay
Ginkgo biloba
-
radicle
5 embryos are pooled to prepare a RNA sample, used for complementation assay
Ginkgo biloba
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ATP
-
689692
Ginkgo biloba
2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ADP
-
-
-
ir
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Ginkgo biloba
theoretical value, predicted
-
5.1
Ginkgo biloba
theoretical value, predicted
-
5.2
Other publictions for EC 2.7.1.148
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739765
Tong
Cloning and expression analysi ...
Tripterygium wilfordii
Zhongguo Zhong Yao Za Zhi
40
4165-4170
2015
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1
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1
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721831
Tang
Identification of novel small ...
Escherichia coli, Escherichia coli DH5-alpha, Yersinia pestis
Bioorg. Med. Chem.
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2011
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2
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8
2
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22
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3
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8
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8
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2
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Crystal structure of 4-diphosp ...
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2011
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2
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721187
Kalinowska-Tluscik
A triclinic crystal form of Es ...
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Acta Crystallogr. Sect. F
66
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2010
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5
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1
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699633
Gimenez-Oya
Mimicking direct protein-prote ...
Escherichia coli
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15
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2009
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1
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1
1
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2
2
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706809
Battilana
The 1-deoxy-D: -xylulose 5-pho ...
Vitis vinifera x Vitis riparia, Vitis vinifera x Vitis vinifera
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2009
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1
2
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1
1
1
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1
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1
1
1
1
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2
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8
1
1
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1
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1
2
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1
1
1
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1
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-
-
-
-
-
-
689692
Kim
Two copies of 4-(cytidine 5-di ...
Ginkgo biloba
Planta
228
941-950
2008
2
-
1
-
-
-
-
-
3
-
2
2
-
6
-
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-
1
-
2
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2
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1
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2
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2
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2
2
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4
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2
2
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4
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2
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2
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673095
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Nonphosphate inhibitors of Isp ...
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ChemMedChem
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806-810
2007
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11
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1
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11
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11
11
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1
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2
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672138
Lherbet
Absence of substrate channelin ...
Agrobacterium tumefaciens
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1
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1
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2
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662149
Wada
Crystal structure of 4-(cytidi ...
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278
30022-30027
2003
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1
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6
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1
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6
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1
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Miallau
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2003
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349728
Rohdich
Biosynthesis of terpenoids: 4- ...
Solanum lycopersicum
Proc. Natl. Acad. Sci. USA
97
8251-8256
2000
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1
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349729
Kuzuyama
-
Studies on the nonmevalonate p ...
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