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Literature summary for 2.7.1.148 extracted from

  • Kim, S.M.; Kim, Y.B.; Kuzuyama, T.; Kim, S.U.
    Two copies of 4-(cytidine 5-diphospho)-2-C-methyl-D: -erythritol kinase (CMK) gene in Ginkgo biloba: molecular cloning and functional characterization (2008), Planta, 228, 941-950.
    View publication on PubMed
Search for more literature for 2.7.1.148

Activating Compound

Activating Compound Comment Organism Structure
light Selective increase in the transcript abundance of GbCMK2 in the radicles by induction of light. Ginkgo biloba
methyl jasmonate Selective increase in the transcript abundance of GbCMK2 in adicles. Ginkgo biloba

Cloned(Commentary)

Cloned (Comment) Organism
The two CMK sequences encoding the mature CMK proteins are amplified with their respective primer pairs and cloned to the pMW118 vector to derive the pMW-CMK1 and the pMW-CMK2 plasmids. NMW29, an Escherichia coli ychB mutant harboring the pTMV20KM plasmid. The strain is transformed with the pMW-CMK1 and the pMW-CMK2 plasmids and selected on the LB medium without mevalonate. Ginkgo biloba

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast isoenzyme contains chloroplast transit peptide sequence Ginkgo biloba 9507
-
cytosol
-
 Ginkgo biloba 5829
-
nucleus
-
 Ginkgo biloba 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39200
-
 theoretical value, predicted Ginkgo biloba
39900
-
 theoretical value, predicted Ginkgo biloba

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ATP Ginkgo biloba
-
 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ADP
-
 ir

Organism

Organism UniProt Comment Textmining
Ginkgo biloba A6XAA8
-
-
Ginkgo biloba A6XAA9
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol catalyzes the fourth step of 2-C-methyl-D-erythritol 4-phosphate pathway Ginkgo biloba
a

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon 5 embryos are pooled to prepare a RNA sample, used for complementation assay Ginkgo biloba
-
radicle 5 embryos are pooled to prepare a RNA sample, used for complementation assay Ginkgo biloba
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ATP
-
 Ginkgo biloba 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ADP
-
 ir

Synonyms

Synonyms Comment Organism
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase
-
 Ginkgo biloba
CDP-ME kinase
-
 Ginkgo biloba
CMK
-
 Ginkgo biloba
GbCMK1 Isoenzyme with 433 amino acids. The identity between GbCMK2 and GbCMK1 is 62.7%. Ginkgo biloba
GbCMK2 isoenzyme with 428 amino acids. The identity between GbCMK2 and GbCMK1 is 62.7%. Ginkgo biloba
YchB
-
 Ginkgo biloba

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Ginkgo biloba

pI Value

Organism Comment pI Value Maximum pI Value
Ginkgo biloba theoretical value, predicted
-
 5.1
Ginkgo biloba theoretical value, predicted
-
 5.2