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Literature summary for 2.7.1.148 extracted from

  • Miallau, L.; Alphey, M.S.; Kemp, L.E.; Leonard, G.A.; McSweeney, S.M.; Hecht, S.; Bacher, A.; Eisenreich, W.; Rohdich, F.; Hunter, W.N.
    Biosynthesis of isoprenoids: crystal structure of 4-diphosphocytidyl-2C-methyl-D-erythritol kinase (2003), Proc. Natl. Acad. Sci. USA, 100, 9173-9178.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene ispE, expression of the His-tagged in strain BL21(DE3) Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme in ternary complex with substrate and non-hydrolyzable ATP analogue adenosine 5'-[beta,gamma-imino]triphosphate, i.e. AMP-PNP, 25 mg/ml protein in 50 mM Tris-HCl, pH 7.7, 50 mM NaCl, 3 mM AMP-PNP, and 2 mM substrate 4-(cytidine 5'-phosphate)-2-C-methyl-D-erythritol, hanging drop vapour diffusion method, 0.001 ml protein solution mixed with equal volume of reservoir solution containing 20% polyethylene glycol 8000, 0.2 M magnesium acetate, 0.1 M sodium cacodylate, pH 6.5, and 0.0002 ml of 0.25 sulfo-betaine, cryoprotection at -173°C by 20% glycerol, X-ray diffraction structure determination and analysis at 2.0 A resolution Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol Escherichia coli the enzyme is essential in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
gene ispE
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from strain BL21(DE3) to homogeneity by nickel affinity and anion exchange chromatography Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol reaction mechanism Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol the enzyme is essential in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis Escherichia coli ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
?
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol i.e. CDP-ME Escherichia coli ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
?

Subunits

Subunits Comment Organism
More the enzyme subunit displays the alpha/beta fold characteristic of the galactose kinase/homoserine kinase/mevalonate kinase phosphomevalonate kinase superfamily, arranged into cofactor and substrate-binding domains with the catalytic center positioned in a deep cleft between domains, quarternary structure, overview Escherichia coli

Synonyms

Synonyms Comment Organism
4-diphosphocytidyl-2C-methyl-D-erythritol kinase
-
Escherichia coli
More the enzyme belongs to the galactose kinase/homoserine kinase/mevalonate kinase phosphomevalonate kinase superfamily Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli