Literature summary for 2.7.1.148 extracted from

Wada, T.; Kuzuyama, T.; Satoh, S.; Kuramitsu, S.; Yokoyama, S.; Unzai, S.; Tame, J.R.; Park, S.Y.
Crystal structure of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, an enzyme in the non-mevalonate pathway of isoprenoid synthesis (2003), J. Biol. Chem., 278, 30022-30027.

Search for more literature for 2.7.1.148

Application

Application	Comment	Organism
drug development	enzyme is a target for antimicrobial drug development	Thermus thermophilus

Cloned(Commentary)

Cloned (Comment)	Organism
gene ychB, expression of the selenomethionine-labeled enzyme in Escherichia coli strain B834(DE3)	Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 20°C, 0.002 ml protein solution containing 2.2 mg/ml protein, 20 ml Tris-HCl, pH 8.0, and 50 mM NaCl, are mixed with 0.001 ml mother liquor containing 33 mM Tris-HCl, pH 8.5, 67 mM sodium acetate, 13% isopropanol, 8% butanol, and 13% PEG 4000, cryoprotection at -173°C in 30% glycerol, X-ray diffraction structure determination and analysis at 1.7 A resolution	Thermus thermophilus

Crystallization (Comment)

Organism

purified recombinant selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 20°C, 0.002 ml protein solution containing 2.2 mg/ml protein, 20 ml Tris-HCl, pH 8.0, and 50 mM NaCl, are mixed with 0.001 ml mother liquor containing 33 mM Tris-HCl, pH 8.5, 67 mM sodium acetate, 13% isopropanol, 8% butanol, and 13% PEG 4000, cryoprotection at -173°C in 30% glycerol, X-ray diffraction structure determination and analysis at 1.7 A resolution

Thermus thermophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	binding site structure, S95, D125, and G94 are important	Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	Thermus thermophilus	the enzyme is essential in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis, pathway overview	ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	Thermus thermophilus HB8 / ATCC 27634 / DSM 579	the enzyme is essential in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis, pathway overview	ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	-	-	-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant selenomethionine-labeled enzyme from Escherichia coli strain B834(DE3) by ammonium sulfate fractionation, hydrophobic interaction and heparin affinity chromatography, gel filtration, and ultrafiltration	Thermus thermophilus

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	reaction mechanism involves K8 and D125	Thermus thermophilus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	the enzyme is essential in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis, pathway overview	Thermus thermophilus	ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	i.e. CDP-ME, the cytosine group plays an essential role in substrate recognition by the enzyme	Thermus thermophilus	ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	the enzyme is essential in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis, pathway overview	Thermus thermophilus HB8 / ATCC 27634 / DSM 579	ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	i.e. CDP-ME, the cytosine group plays an essential role in substrate recognition by the enzyme	Thermus thermophilus HB8 / ATCC 27634 / DSM 579	ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?
additional information	no activity with 4-(adenine 5'-phosphate)-2-C-methyl-D-erythritol and 4-(uridine 5'-phosphate)-2-C-methyl-D-erythritol	Thermus thermophilus	?	-	?
additional information	no activity with 4-(adenine 5'-phosphate)-2-C-methyl-D-erythritol and 4-(uridine 5'-phosphate)-2-C-methyl-D-erythritol	Thermus thermophilus HB8 / ATCC 27634 / DSM 579	?	-	?

Synonyms

Synonyms	Comment	Organism
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-	Thermus thermophilus
CDP-ME kinase	-	Thermus thermophilus
CMK	-	Thermus thermophilus
More	enzyme belongs to the GHMK super family	Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	assay at	Thermus thermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Thermus thermophilus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	binding site structure, N58 and K83 are important	Thermus thermophilus