Cloned (Comment) | Organism |
---|---|
gene ychB, overexpression of His6-tagged enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | Escherichia coli | fourth reaction step of the 2-C-methyl-D-erythritol 4-phosphate pathway | ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme by nickel affinity chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
spectrophotometric and HPLC-based assay development for determination of product ADP with use of pyruvate kinase and L-lactate dehydrogenase as auxiliary enzymes | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | fourth reaction step of the 2-C-methyl-D-erythritol 4-phosphate pathway | Escherichia coli | ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | i.e. CDP-ME | Escherichia coli | ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 37 | assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at, spectrophotometric assay | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |