Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.148 extracted from

  • Bernal, C.; Mendez, E.; Terencio, J.; Boronat, A.; Imperial, S.
    A spectrophotometric assay for the determination of 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase activity (2005), Anal. Biochem., 340, 245-251.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ychB, overexpression of His6-tagged enzyme Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol Escherichia coli fourth reaction step of the 2-C-methyl-D-erythritol 4-phosphate pathway ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme by nickel affinity chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
spectrophotometric and HPLC-based assay development for determination of product ADP with use of pyruvate kinase and L-lactate dehydrogenase as auxiliary enzymes Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol fourth reaction step of the 2-C-methyl-D-erythritol 4-phosphate pathway Escherichia coli ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
?
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol i.e. CDP-ME Escherichia coli ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
?

Synonyms

Synonyms Comment Organism
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 37 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at, spectrophotometric assay Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli