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Literature summary for 2.7.1.147 extracted from

  • Grudnik, P.; Kaminski, M.M.; Rembacz, K.P.; Kuska, K.; Madej, M.; Potempa, J.; Dawidowski, M.; Dubin, G.
    Structural basis for ADP-dependent glucokinase inhibition by 8-bromo-substituted adenosine nucleotide (2018), J. Biol. Chem., 293, 11088-11099 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.147

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens
expression in Escherichia coli Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method at 20°C, complex of the enzyme with glucose and 8-bromoadenosine phosphate Homo sapiens
sitting drop vapor diffusion method at 20°C, complex of the enzyme with glucose and 8-bromoadenosine phosphate Pyrococcus horikoshii

Inhibitors

Inhibitors Comment Organism Structure
8-bromoadenosine phosphate competitive. The binding of the inhibitor induces major conformational changes Homo sapiens
8-bromoadenosine phosphate competitive Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 ADP pH 7.4, 37°C Homo sapiens
0.13
-
 ADP pH 7.4, 37°C Pyrococcus horikoshii
0.25
-
 D-glucose pH 7.4, 37°C Homo sapiens
0.64
-
 D-glucose pH 7.4, 37°C Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + D-glucose Homo sapiens
-
 AMP + D-glucose 6-phosphate
-
 ?
ADP + D-glucose Pyrococcus horikoshii
-
 AMP + D-glucose 6-phosphate
-
 ?
ADP + D-glucose Pyrococcus horikoshii ATCC 700860
-
 AMP + D-glucose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9BRR6
-
-
Pyrococcus horikoshii O58328
-
-
Pyrococcus horikoshii ATCC 700860 O58328
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + D-glucose
-
 Homo sapiens AMP + D-glucose 6-phosphate
-
 ?
ADP + D-glucose
-
 Pyrococcus horikoshii AMP + D-glucose 6-phosphate
-
 ?
ADP + D-glucose
-
 Pyrococcus horikoshii ATCC 700860 AMP + D-glucose 6-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
ADP-dependent glucokinase
-
 Homo sapiens
ADP-dependent glucokinase
-
 Pyrococcus horikoshii
ADPGK
-
 Homo sapiens
ADPGK
-
 Pyrococcus horikoshii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.16
-
 8-bromoadenosine phosphate pH 7.4, 37°C Pyrococcus horikoshii
0.27
-
 8-bromoadenosine phosphate pH 7.4, 37°C Homo sapiens