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Literature summary for 2.7.1.147 extracted from

  • Richter, S.; Morrison, S.; Connor, T.; Su, J.; Print, C.G.; Ronimus, R.S.; McGee, S.L.; Wilson, W.R.
    Zinc finger nuclease mediated knockout of ADP-dependent glucokinase in cancer cell lines: effects on cell survival and mitochondrial oxidative metabolism (2013), PLoS ONE, 8, e65267.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.147

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
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Source Tissue

Source Tissue Comment Organism Textmining
H-460 cell
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 Homo sapiens
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HCT-116 cell
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 Homo sapiens
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General Information

General Information Comment Organism
physiological function knockout of inc finger nucleases in each H-460 and HCT-11 cells lead to frameshift mutations in all alleles at the target site in exon 1 of ADP-dependent glucokinase ADPGK, and are ADPGK-null by immunoblotting. ADPGK knockout has little or noeffect on cell proliferation, but compromises the ability of H-460 cells to survive siRNA silencing of hexokinase-2 under oxic conditions and anoxia. No such changes are found when ADPGK is knocked out in HCT-116 cells. Knockout of ADPGK in HCT-116 cells causes few changes in global gene expression, while knockout of ADPGK in H-460 cells causes notable up-regulation of mRNAs encoding cell adhesion proteins. No consistent effect on glycolysis under oxic conditions in a variety of media is observed. Oxygen consumption rates are generally lower in the ADPGK knockouts Homo sapiens