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Literature summary for 2.7.1.147 extracted from

  • Rivas-Pardo, J.A.; Herrera-Morande, A.; Castro-Fernandez, V.; Fernandez, F.J.; Vega, M.C.; Guixe, V.
    Crystal structure, SAXS and kinetic mechanism of hyperthermophilic ADP-dependent glucokinase from Thermococcus litoralis reveal a conserved mechanism for catalysis (2013), PLoS One, 8, e66687.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.147

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of apo form and holo form, in the presence of D-glucose and the nonhydrolyzable ADP analog adenosine 5'-(3-thio)diphosphate. The conformational changes upon sequential substrate binding can be explained by an almost pure rotation (or a rotation plus a translation) facilitated by residues in the flexible inter-domain connection Thermococcus litoralis
sitting-drop vapor-diffusion crystallization Thermococcus litoralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0086
-
 ADP pH not specified in the publication, temperature not specified in the publication Thermococcus litoralis
0.0086
-
 ADP 40°C, pH 7.8 Thermococcus litoralis
0.218
-
 D-glucose pH not specified in the publication, temperature not specified in the publication Thermococcus litoralis
0.219
-
 D-glucose 40°C, pH 7.8 Thermococcus litoralis

Organism

Organism UniProt Comment Textmining
Thermococcus litoralis Q7M537
-
-
Thermococcus litoralis DSM 5473 Q7M537
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermococcus litoralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + D-glucose
-
 Thermococcus litoralis AMP + D-glucose 6-phosphate
-
 ?
ADP + D-glucose catalysis follows a sequential ordered mechanism Thermococcus litoralis AMP + D-glucose 6-phosphate
-
 ?
ADP + D-glucose
-
 Thermococcus litoralis DSM 5473 AMP + D-glucose 6-phosphate
-
 ?
ADP + D-glucose catalysis follows a sequential ordered mechanism Thermococcus litoralis DSM 5473 AMP + D-glucose 6-phosphate
-
 ?
additional information catalysis follows a sequential ordered mechanism Thermococcus litoralis ?
-
 ?
additional information catalysis follows a sequential ordered mechanism Thermococcus litoralis DSM 5473 ?
-
 ?

Synonyms

Synonyms Comment Organism
ADP-dependent glucokinase
-
 Thermococcus litoralis