Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.147 extracted from

  • Tsuge, H.; Sakuraba, H.; Kobe, T.; Kujime, A.; Katunuma, N.; Ohshima, T.
    Crystal structure of the ADP-dependent glucokinase from Pyrococcus horikoshii at 2.0-A resolution: a large conformational change in ADP-dependent glucokinase (2002), Protein Sci., 11, 2456-2463.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene PH0589, DNA and amino acid sequence determination and analysis, expression of the N-terminally His-tagged in Escherichia coli Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant N-terminally His-tagged apoenzyme, hanging drop vapour diffusion method, 20°C, 10 mg/ml protein in solution is mixed with an equal volume of 0.003 ml of mother liquor containing 9-13% PEG 6000, 0.2 M Li2SO4, and 0.1 M citrate buffer, pH 3.6, heavy atom derivatization with HgCl2, X-ray diffraction structure determination and analysis at 2.0 A resolution, single isomorphous replacement with an anomalous scattering Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + D-glucose Pyrococcus horikoshii
-
AMP + D-glucose 6-phosphate
-
?
ADP + D-glucose Pyrococcus horikoshii OT-3
-
AMP + D-glucose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
strain OT3, gene PH0589
-
Pyrococcus horikoshii OT-3
-
strain OT3, gene PH0589
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli by heat treatment and nickel affinity chromatography Pyrococcus horikoshii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity determination in a coupled assay with glucose 6-phosphate dehydrogenase and NADP+ Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + D-glucose
-
Pyrococcus horikoshii AMP + D-glucose 6-phosphate
-
?
ADP + D-glucose
-
Pyrococcus horikoshii OT-3 AMP + D-glucose 6-phosphate
-
?
additional information substrate-induced fit, binding of ADP induces large conformational changes Pyrococcus horikoshii ?
-
?
additional information substrate-induced fit, binding of ADP induces large conformational changes Pyrococcus horikoshii OT-3 ?
-
?

Synonyms

Synonyms Comment Organism
ADP-dependent glucokinase
-
Pyrococcus horikoshii
ADPGK
-
Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
assay at Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Pyrococcus horikoshii

Cofactor

Cofactor Comment Organism Structure
ADP binding site structure composed of 2 loops Pyrococcus horikoshii