Literature summary for 2.7.1.146 extracted from

Tuininga, J.E.; Verhees, C.H.; van der Oost, J.; Kengen, S.W.; Stams, A.J.; de Vos, W.M.
Molecular and biochemical characterization of the ADP-dependent phosphofructokinase from the hyperthermophilic archaeon Pyrococcus furiosus (1999), J. Biol. Chem., 274, 21023-21028.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pyrococcus furiosus

Inhibitors

Inhibitors	Comment	Organism	Structure
AMP	competitive inhibition	Pyrococcus furiosus
ATP	competitive inhibition	Pyrococcus furiosus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	81% of activity	Pyrococcus furiosus
Mg2+	required for activity	Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
52000	-	4 * 52000, SDS-PAGE	Pyrococcus furiosus
180000	-	gel filtration	Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-fructose 6-phosphate + ADP	Pyrococcus furiosus	ADP can be replaced by GDP, ATP and GTP to a limited extent	D-fructose 1,6-bisphosphate + AMP	-	ir

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partial	Pyrococcus furiosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
88	-	-	Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose 6-phosphate + ADP	ADP can be replaced by GDP, ATP and GTP to a limited extent	Pyrococcus furiosus	D-fructose 1,6-bisphosphate + AMP	-	ir

Subunits

Subunits	Comment	Organism
tetramer	4 * 52000, SDS-PAGE	Pyrococcus furiosus

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Release 2023.1 (January 2023)