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Literature summary for 2.7.1.145 extracted from
- Non-viral deoxyribonucleoside kinases - diversity and practical use (2015), J. Genet. Genomics, 42, 235-248.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | suicide gene dmdnk in several cancer cell lines, overview. In vivo testing in a xenograft model for breast cancer, both wild-type DmdNK and mutants of DmdNK are combined with gemcitabine (dFdC, 2',2'-difluoro-deoxycytidine) as the NA prodrug and a positive effect of decreasing tumor volume and increased survival time | Drosophila melanogaster |
| additional information | DmdNK broad substrate specificity and fast turnover rate make it an interesting candidate for suicide gene therapy, as well as in numerous biotechnological applications | Drosophila melanogaster |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure determination, PDB IDs 1OE0, 1OT3, 1J90, 1ZM7, 1ZMX, 2JCS, 2VP0, 2VP2, 2VP4, 2VP, 2VP6, 2VP9, 2VPP, 2VQ, and 2JJ8 | Drosophila melanogaster |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Bombyx mori | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Anopheles gambiae |  |
| Mg2+ | required | Drosophila melanogaster | |
| Mg2+ | required | Bombyx mori | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2'-deoxyadenosine | Anopheles gambiae | - |
ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyadenosine | Drosophila melanogaster | - |
ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyadenosine | Bombyx mori | - |
ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxycytidine | Anopheles gambiae | - |
ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxycytidine | Drosophila melanogaster | - |
ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxycytidine | Bombyx mori | - |
ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyguanosine | Anopheles gambiae | - |
ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyguanosine | Drosophila melanogaster | - |
ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyguanosine | Bombyx mori | - |
ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyribonucleoside | Anopheles gambiae | - |
ADP + 2'-deoxyribonucleoside 5'-phosphate | - |
? | |
| ATP + 2'-deoxyribonucleoside | Drosophila melanogaster | - |
ADP + 2'-deoxyribonucleoside 5'-phosphate | - |
? | |
| ATP + 2'-deoxyribonucleoside | Bombyx mori | - |
ADP + 2'-deoxyribonucleoside 5'-phosphate | - |
? | |
| ATP + 2'-deoxythymidine | Anopheles gambiae | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxythymidine | Drosophila melanogaster | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxythymidine | Bombyx mori | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
| additional information | Bombyx mori | the multisubstrate kinase from Bombyx mori (BmdNK) prefers pyrimidines | ? | - |
? | |
| additional information | Drosophila melanogaster | the multisubstrate kinase from Drosophila prefers pyrimidines | ? | - |
? | |
| additional information | Anopheles gambiae | the multisubstrate kinase from from Anopheles gambiae (AgdNK) prefers purines | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anopheles gambiae | Q86LB8 | gene dnk | - |
| Bombyx mori | Q9BKL3 | gene dnk | - |
| Drosophila melanogaster | Q9XZT6 | gene dnk | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2'-deoxyadenosine | - |
Anopheles gambiae | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyadenosine | - |
Drosophila melanogaster | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyadenosine | - |
Bombyx mori | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyadenosine | 1% of the activity with 2'-deoxythymidine | Drosophila melanogaster | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyadenosine | 2.7% of the activity with 2'-deoxycytidine | Bombyx mori | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyadenosine | 48% of the activity with 2'-deoxythymidine | Anopheles gambiae | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxycytidine | - |
Anopheles gambiae | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxycytidine | - |
Drosophila melanogaster | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxycytidine | - |
Bombyx mori | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxycytidine | best substrate | Bombyx mori | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxycytidine | 88% of the activity with 2'-deoxythymidine | Drosophila melanogaster | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxycytidine | 99% of the activity with 2'-deoxythymidine | Anopheles gambiae | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyguanosine | - |
Anopheles gambiae | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyguanosine | - |
Drosophila melanogaster | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyguanosine | - |
Bombyx mori | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyguanosine | 0.2% of the activity with 2'-deoxythymidine | Drosophila melanogaster | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyguanosine | 0.5% of the activity with 2'-deoxycytidine | Bombyx mori | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyguanosine | 83% of the activity with 2'-deoxythymidine | Anopheles gambiae | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyribonucleoside | - |
Anopheles gambiae | ADP + 2'-deoxyribonucleoside 5'-phosphate | - |
? | |
| ATP + 2'-deoxyribonucleoside | - |
Drosophila melanogaster | ADP + 2'-deoxyribonucleoside 5'-phosphate | - |
? | |
| ATP + 2'-deoxyribonucleoside | - |
Bombyx mori | ADP + 2'-deoxyribonucleoside 5'-phosphate | - |
? | |
| ATP + 2'-deoxythymidine | - |
Anopheles gambiae | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxythymidine | - |
Drosophila melanogaster | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxythymidine | - |
Bombyx mori | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxythymidine | best substrate | Anopheles gambiae | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxythymidine | best substrate | Drosophila melanogaster | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxythymidine | 65% of the activity with 2'-deoxycytidine | Bombyx mori | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
| additional information | the multisubstrate kinase from Bombyx mori (BmdNK) prefers pyrimidines | Bombyx mori | ? | - |
? | |
| additional information | the multisubstrate kinase from Drosophila prefers pyrimidines | Drosophila melanogaster | ? | - |
? | |
| additional information | the multisubstrate kinase from from Anopheles gambiae (AgdNK) prefers purines | Anopheles gambiae | ? | - |
? | |
| additional information | substrate binding of non-TK1-like dNKs, overview | Anopheles gambiae | ? | - |
? | |
| additional information | substrate binding of non-TK1-like dNKs, overview | Drosophila melanogaster | ? | - |
? | |
| additional information | substrate binding of non-TK1-like dNKs, overview | Bombyx mori | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AgdNK | - |
Anopheles gambiae |
| BmdNK | - |
Bombyx mori |
| DmdNK | - |
Drosophila melanogaster |
| dNK | - |
Anopheles gambiae |
| dNK | - |
Drosophila melanogaster |
| dNK | - |
Bombyx mori |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Anopheles gambiae | |
| ATP | - |
Drosophila melanogaster | |
| ATP | - |
Bombyx mori | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | evolution of dNKs, overview. The two families of dNKs, the TK1-like kinases and the non-TK1-like kinases, have overall unique monomer structures and only share significant protein sequence similarity at the Ploop, a glycine rich ATP binding domain close to the N-terminus. This suggests that the two families have different evolutionary origins. The insect dNK is a non-TK1 like kinase | Anopheles gambiae |
| evolution | evolution of dNKs, overview. The two families of dNKs, the TK1-like kinases and the non-TK1-like kinases, have overall unique monomer structures and only share significant protein sequence similarity at the Ploop, a glycine rich ATP binding domain close to the N-terminus. This suggests that the two families have different evolutionary origins. The insect dNK is a non-TK1 like kinase | Drosophila melanogaster |
| evolution | evolution of dNKs, overview. The two families of dNKs, the TK1-like kinases and the non-TK1-like kinases, have overall unique monomer structures and only share significant protein sequence similarity at the Ploop, a glycine rich ATP binding domain close to the N-terminus. This suggests that the two families have different evolutionary origins. The insect dNK is a non-TK1 like kinase | Bombyx mori |
| additional information | structure of non-TK1 like kinases, overview | Anopheles gambiae |
| additional information | structure of non-TK1 like kinases, overview | Drosophila melanogaster |
| additional information | structure of non-TK1 like kinases, overview | Bombyx mori |
| physiological function | DmdNK is cell cycle regulated and under the control of cyclin E and the dE2F1 transcription factor, involved in G1/S phase transition | Drosophila melanogaster |
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Release 2023.1 (January 2023)
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