Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.145 extracted from

  • Serra, I.; Conti, S.; Piškur, J.; Clausen, A.; Munch-Petersen, B.; Terreni, M.; Ubiali, D.
    Immobilized Drosophila melanogaster deoxyribonucleoside kinase (DmdNK) as a high performing biocatalyst for the synthesis of purine arabinonucleotides (2014), Adv. Synth. Catal., 356, 563-570.
No PubMed abstract available

Application

Application Comment Organism
synthesis the cross-linked DmdNK preparation is used for the preparative synthesis of arabinosyladenine monophosphate (ara-AMP) and fludarabine monophosphate (FaraAMP) Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
additional information enzyme DmdNK is adsorbed on a solid ion exchange support (bearing primary amino groups) achieving an expressed activity of over 98%. Upon cross-linking with aldehyde dextran, the expressed activity is 30-40%, both biocatalysts (adsorbed or cross-linked) are stable at pH 10.0 and room temperature for 24 h with about 70% of retained activity. Optimization of the reaction conditions to 50 mM ammonium acetate, a substrate/ATP ratio of 1:1.25, 2 mM MgCl2, 37°C, pH 8.0, results in conversions of up to 90% Drosophila melanogaster

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2'-deoxyribonucleoside Drosophila melanogaster
-
ADP + 2'-deoxyribonucleoside 5'-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q9XZT6 gene dnk
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxycytidine
-
Drosophila melanogaster ADP + 2'-deoxycytidine 5'-phosphate
-
?
ATP + 2'-deoxyguanosine
-
Drosophila melanogaster ADP + 2'-deoxyguanosine 5'-phosphate
-
?
ATP + 2'-deoxyinosine
-
Drosophila melanogaster ADP + 2'-deoxyinosine 5'-phosphate
-
?
ATP + 2'-deoxyribonucleoside
-
Drosophila melanogaster ADP + 2'-deoxyribonucleoside 5'-phosphate
-
?
ATP + adenosine low activity Drosophila melanogaster ADP + adenosine 5'-phosphate
-
?
ATP + arabinosyl-2-fluoroadenine
-
Drosophila melanogaster ADP + arabinosyl-2-fluoroadenine 5'-monophosphate
-
?
ATP + arabinosyladenine
-
Drosophila melanogaster ADP + arabinosyladenine 5'-monophosphate
-
?
ATP + arabinosyluridine
-
Drosophila melanogaster ADP + arabinosyluridine 5'-monophosphate
-
?
ATP + cytidine
-
Drosophila melanogaster ADP + cytidine 5'-phosphate
-
?
ATP + guanosine low activity Drosophila melanogaster ADP + guanosine 5'-phosphate
-
?
ATP + inosine very low activity Drosophila melanogaster ADP + inosine 5'-phosphate
-
?
ATP + thymidine
-
Drosophila melanogaster ADP + thymidine 5'-phosphate
-
?
ATP + uridine very low activity Drosophila melanogaster ADP + uridine 5'-phosphate
-
?
additional information enzyme DmdNK is able to phosphorylate all four natural 2'-deoxyribonucleosides with high efficiency, it shows broad specificity and high turnover towards the naturally occurring 2'-deoxyribonucleosides and a similar broad specificity towards nucleoside analogues used in chemotherapy, substrate specificity towards natural and non-natural nucleosides, overview Drosophila melanogaster ?
-
?

Synonyms

Synonyms Comment Organism
DmdNK
-
Drosophila melanogaster

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
immobilized enzyme Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
immobilized enzyme Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
ATP
-
Drosophila melanogaster