Any feedback?
Literature summary for 2.7.1.145 extracted from
- Deoxyribonucleoside kinases belonging to the thymidine kinase 2 (TK2)-like group vary significantly in substrate specificity, kinetics and feed-back regulation (2002), J. Mol. Biol., 315, 529-540.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as C-terminally His8-tagged protein in Escherichia coli strain BL21 | Bombyx mori |
| DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as full length and N-terminally truncated His-tagged enzymes in Escherichia coli | Xenopus laevis |
| DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of wild-type and as C-terminally His8-tagged protein in Escherichia coli strain BL21 | Drosophila melanogaster |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dCTP | competitive versus ATP, feedback inhibition | Xenopus laevis |  |
| dTTP | competitive versus ATP, feedback inhibition | Bombyx mori | |
| dTTP | competitive versus ATP, feedback inhibition | Drosophila melanogaster | |
| dTTP | competitive versus ATP, feedback inhibition | Xenopus laevis | |
| additional information | no inhibition by dNTPs other than dTTP | Bombyx mori | |
| additional information | no inhibition by dNTPs other than dTTP | Drosophila melanogaster |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Bombyx mori | |
| additional information | - |
additional information | kinetics | Xenopus laevis | |
| 0.0014 | - |
ATP | - |
Bombyx mori | |
| 0.0025 | - |
ATP | - |
Xenopus laevis | |
| 0.0061 | - |
2'-deoxycytidine | - |
Bombyx mori | |
| 0.0153 | - |
2'-deoxythymidine | - |
Bombyx mori | |
| 0.031 | - |
2'-deoxycytidine | - |
Xenopus laevis | |
| 0.138 | - |
2'-deoxythymidine | - |
Xenopus laevis | |
| 0.429 | - |
2'-deoxyguanosine | - |
Bombyx mori | |
| 0.501 | - |
2'-deoxyadenosine | - |
Bombyx mori | |
| 1.2 | - |
2'-deoxyguanosine | - |
Xenopus laevis | |
| 2.1 | - |
2'-deoxyadenosine | - |
Xenopus laevis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | no sorting signals in the amino acid sequence | Bombyx mori | - |
- |
| nucleus | enzyme possesses 2 potential nuclear targeting sequences | Drosophila melanogaster | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Bombyx mori | |
| Mg2+ | - |
Xenopus laevis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26300 | - |
2 * 26300, recombinant enzyme, SDS-PAGE | Xenopus laevis |
| 26900 | - |
2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE | Drosophila melanogaster |
| 29100 | - |
2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE | Drosophila melanogaster |
| 31300 | - |
2 * 31300, recombinant enzyme, SDS-PAGE | Bombyx mori |
| 44600 | - |
recombinant N-terminally truncated enzyme, gel filtration | Drosophila melanogaster |
| 46000 | - |
recombinant enzyme, native PAGE | Xenopus laevis |
| 48500 | - |
recombinant full length enzyme, gel filtration | Drosophila melanogaster |
| 49000 | - |
recombinant N-terminally truncated enzyme, native PAGE | Drosophila melanogaster |
| 49800 | - |
recombinant enzyme, gel filtration | Xenopus laevis |
| 57700 | - |
recombinant full length enzyme, native PAGE | Drosophila melanogaster |
| 64400 | - |
recombinant enzyme, gel filtration | Bombyx mori |
| 69300 | - |
recombinant enzyme, native PAGE | Bombyx mori |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2'-deoxycytidine | Xenopus laevis | - |
ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxythymidine | Xenopus laevis | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombyx mori | Q9BKL3 | putative | - |
| Drosophila melanogaster | - |
- |
- |
| Xenopus laevis | Q8UVZ9 | putative | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography | Bombyx mori |
| recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography, the His-tag is cleaved off by thrombin | Drosophila melanogaster |
| recombinant full length and N-terminally truncated His-tagged enzymes, the His-tags are cleaved off | Xenopus laevis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Xenopus laevis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate specificity | Bombyx mori |
| additional information | - |
substrate specificity | Xenopus laevis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1-beta-D-arabinofuranosylcytosine | - |
Bombyx mori | ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate | - |
? | |
| ATP + 1-beta-D-arabinofuranosylcytosine | - |
Xenopus laevis | ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate | - |
? | |
| ATP + 1-beta-D-arabinofuranosylthymine | - |
Bombyx mori | ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate | - |
? | |
| ATP + 1-beta-D-arabinofuranosylthymine | - |
Xenopus laevis | ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate | - |
? | |
| ATP + 2',3'-didehydro-3'-deoxythymidine | - |
Bombyx mori | ADP + 2',3'-didehydro-3'-deoxythymidine 5'-phosphate | - |
? | |
| ATP + 2',3'-dideoxycytidine | - |
Bombyx mori | ADP + 2',3'-dideoxycytidine 5'-phosphate | - |
? | |
| ATP + 2',3'-dideoxycytidine | low activity | Xenopus laevis | ADP + 2',3'-dideoxycytidine 5'-phosphate | - |
? | |
| ATP + 2',3'-dideoxythymidine | - |
Bombyx mori | ADP + 2',3'-dideoxythymidine 5'-phosphate | - |
? | |
| ATP + 2',3'-dideoxythymidine | low activity | Xenopus laevis | ADP + 2',3'-dideoxythymidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyadenosine | - |
Bombyx mori | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyadenosine | - |
Xenopus laevis | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxycytidine | - |
Bombyx mori | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxycytidine | - |
Xenopus laevis | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyguanosine | - |
Bombyx mori | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyguanosine | - |
Xenopus laevis | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxythymidine | - |
Xenopus laevis | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxythymidine | best substrate | Xenopus laevis | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
| ATP + 2'-deoxythymidine | high activity | Bombyx mori | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
| ATP + 2-chloro-2'-deoxyadenosine | low activity | Xenopus laevis | ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 2-chloro-2'-deoxyadenosine | high activity | Bombyx mori | ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine | low activity | Bombyx mori | ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate | - |
? | |
| ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine | low activity | Xenopus laevis | ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate | - |
? | |
| ATP + 3'-deoxyadenosine | - |
Bombyx mori | ADP + 3'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 5-fluoro-2'-deoxyuridine | high activity | Bombyx mori | ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate | - |
? | |
| ATP + 5-fluoro-2'-deoxyuridine | high activity | Xenopus laevis | ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate | - |
? | |
| ATP + 9-beta-D-arabinofuranosyladenine | - |
Bombyx mori | ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate | - |
? | |
| ATP + 9-beta-D-arabinofuranosyladenine | very low activity | Xenopus laevis | ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate | - |
? | |
| ATP + aciclovir | - |
Bombyx mori | ADP + aciclovir phosphate | - |
? | |
| ATP + cytidine | - |
Bombyx mori | ADP + cytidine 5'-phosphate | - |
? | |
| ATP + cytidine | very low activity | Xenopus laevis | ADP + cytidine 5'-phosphate | - |
? | |
| ATP + deoxyuridine | best substrate | Bombyx mori | ADP + deoxyuridine 5'-phosphate | - |
? | |
| ATP + deoxyuridine | high activity | Xenopus laevis | ADP + deoxyuridine 5'-phosphate | - |
? | |
| ATP + fludarabine | - |
Bombyx mori | ADP + fludarabine phosphate | - |
? | |
| ATP + stavudine | anti-HIV nucleoside analogue | Bombyx mori | ADP + stavudine phosphate | - |
? | |
| ATP + uridine | very low activity | Xenopus laevis | ADP + uridine 5'-phosphate | - |
? | |
| ATP + uridine | low activity | Bombyx mori | ADP + uridine 5'-phosphate | - |
? | |
| additional information | substrate specificity | Drosophila melanogaster | ? | - |
? | |
| additional information | substrate specificity, no activity with 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine | Bombyx mori | ? | - |
? | |
| additional information | substrate specificity, no activity with fludarabine, 3'-deoxyadenosine, 2',3'-didehydro-3'-deoxythymidine, 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine | Xenopus laevis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 26300, recombinant enzyme, SDS-PAGE | Xenopus laevis |
| dimer | 2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE | Drosophila melanogaster |
| dimer | 2 * 31300, recombinant enzyme, SDS-PAGE | Bombyx mori |
| More | quarternary structure determination | Bombyx mori |
| More | quarternary structure determination | Xenopus laevis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dNK | - |
Drosophila melanogaster |
| dNK | - |
Bombyx mori |
| More | the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes | Drosophila melanogaster |
| More | the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes | Bombyx mori |
| More | the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes | Xenopus laevis |
| multisubstrate deoxyribonucleoside kinase | - |
Drosophila melanogaster |
| multisubstrate deoxyribonucleoside kinase | - |
Bombyx mori |
| pyrimidine deoxyribonucleoside kinase | - |
Xenopus laevis |
| Xen-PyK | - |
Xenopus laevis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bombyx mori | |
| ATP | - |
Xenopus laevis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics, overview | Drosophila melanogaster | |
| additional information | - |
additional information | inhibition kinetics, overview | Bombyx mori | |
| additional information | - |
additional information | inhibition kinetics, overview | Xenopus laevis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
