Application | Comment | Organism |
---|---|---|
agriculture | multifunctional deoxynucleoside kinase of insect cells is a target for development of new highly selective insecticidial drugs | Drosophila melanogaster |
Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes, expression in Escherichia coli | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(E)-5-(2-bromovinyl)-2'-deoxyuridine | - |
Drosophila melanogaster | |
(E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-monophosphate | IC50: 0.0026 mM, reaction with thymidine, fusion protein with glutathione S-transferase | Drosophila melanogaster | |
(E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-triphosphate | IC50: 0.0069 mM, reaction with thymidine, fusion protein with glutathione S-transferase. The inhibitor is efficiently metabolized to its 5'-mono-, 5'-di- and 5'-triphosphate derivatives in the insect cell culture and abundently incorporated into the insect cell DNA | Drosophila melanogaster | |
dTTP | IC50: 0.012 mM, reaction with thymidine, fusion protein with glutathione S-transferase | Drosophila melanogaster | |
additional information | multifunctional deoxynucleoside kinase of insect cells is a target for development of new highly selective insecticidial drugs | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
(E)-5-(2-bromovinyl)-2'-deoxyuridine | 37°C, fusion protein with glutathione S-transferase | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thymidine + ATP | Drosophila melanogaster | - |
thymidine 5'-phosphate + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-5-(2-bromovinyl)-2'-deoxyuridine + ATP | - |
Drosophila melanogaster | (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate + ADP | - |
? | |
thymidine + ATP | - |
Drosophila melanogaster | thymidine 5'-phosphate + ADP | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00066 | - |
(E)-5-(2-bromovinyl)-2'-deoxyuridine | pH 7.6, 30°C, reaction with thymidine, fusion protein with glutathione S-transferase | Drosophila melanogaster |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0026 | - |
IC50: 0.0026 mM, reaction with thymidine, fusion protein with glutathione S-transferase | Drosophila melanogaster | (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-monophosphate | |
0.0069 | - |
IC50: 0.0069 mM, reaction with thymidine, fusion protein with glutathione S-transferase. The inhibitor is efficiently metabolized to its 5'-mono-, 5'-di- and 5'-triphosphate derivatives in the insect cell culture and abundently incorporated into the insec | Drosophila melanogaster | (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-triphosphate | |
0.012 | - |
IC50: 0.012 mM, reaction with thymidine, fusion protein with glutathione S-transferase | Drosophila melanogaster | dTTP |