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Literature summary for 2.7.1.145 extracted from

  • Johansson, M.; van Rompay, A.R.; Degreve, B.; Balzarini, J.; Karlsson, A.
    Cloning and characterization of the multisubstrate deoxyribonucleoside kinase of Drosophila melanogaster (1999), J. Biol. Chem., 274, 23814-23819.
    View publication on PubMed
Search for more literature for 2.7.1.145

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
(E)-5-(2-bromovinyl)-1-beta-D-arabinofuranosyluracil IC50: 0.0277 mM for reaction with thymidine and 0.0158 mM for reaction with 2'-deoxycytidine Drosophila melanogaster
(E)-5-(2-bromovinyl)-2'-deoxyuridine IC50: 0.00257 mM for reaction with thymidine and 0.00194 mM for reaction with 2'-deoxycytidine Drosophila melanogaster
1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-5-iodouracil IC50: 0.0163 mM for reaction with thymidine and 0.0154 mM for reaction with 2'-deoxycytidine Drosophila melanogaster
1-beta-D-arabinofuranosylcytosine IC50: 0.0534 mM for reaction with thymidine and 0.089 mM for reaction with 2'-deoxycytidine Drosophila melanogaster
1-beta-D-arabinofuranosylthymidine IC50: 0.0648 mM for reaction with thymidine and 0.0348 mM for reaction with 2'-deoxycytidine Drosophila melanogaster
1-beta-D-arabinofuranosyluracil IC50: 0.057 mM for reaction with thymidine and 0.058 mM for reaction with 2'-deoxycytidine Drosophila melanogaster
2',2'-difluorodeoxycytidine IC50: 0.145 mM for reaction with thymidine and 0.102 mM for reaction with 2'-deoxycytidine Drosophila melanogaster
2',3'-dehydro-3'-deoxythymidine IC50 for reaction with thymidine is greater than 1000 mM, IC50 for reaction with 2'-deoxycytidine is 0.805 mM Drosophila melanogaster
2',3'-dideoxycytidine IC50: 0.76 mM for reaction with thymidine and 0.838 mM for reaction with 2'-deoxycytidine Drosophila melanogaster
2',3'-dideoxythymidine IC50: 0.075 mM for reaction with thymidine and 0.0703 mM for reaction with 2'-deoxycytidine Drosophila melanogaster
2'-deoxy-3'-thiacytidine IC50: 0.74 mM for reaction with thymidine and 0.868 mM for reaction with 2'-deoxycytidine Drosophila melanogaster
2'-deoxycytidine competitive with thymidine Drosophila melanogaster
2'-deoxyuridine reaction with thymidine and 2'-deoxycytidine Drosophila melanogaster
2-chloro-2'-deoxyadenosine IC50: 0.123 mM for reaction with thymidine and 0.12 mM for reaction with 2'-deoxycytidine Drosophila melanogaster
3'-azido-2',3'-dideoxythymidine IC50: 0.0419 mM for reaction with thymidine and 0.0296 mM for reaction with 2'-deoxycytidine Drosophila melanogaster
5-fluoro-2'-deoxyuridine IC50: 0.0258 mM for reaction with thymidine and 0.02235 mM for reaction with 2'-deoxycytidine Drosophila melanogaster
thymidine competitive with 2'-deoxycytidine Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 thymidine pH 8, 37°C Drosophila melanogaster
0.0026
-
 2'-deoxycytidine pH 8, 37°C Drosophila melanogaster
0.373
-
 2'-deoxyadenosine pH 8, 37°C Drosophila melanogaster
2
-
 2'-deoxyguanosine pH 8, 37°C Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 1 * 29000, calculation from nucleotide sequence Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q9XZT6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxycytidine + ATP
-
 Drosophila melanogaster 2'-deoxycytidine 5'-phosphate + ADP
-
 ?
additional information the recombinant enzyme preferentially phosphorylates the pyrimidine nucleosides, but phosphorylation of the purine nucleosidesis also efficiently catalyzed Drosophila melanogaster ?
-
 ?
additional information no activity with TMP Drosophila melanogaster ?
-
 ?
thymidine + ATP
-
 Drosophila melanogaster thymidine 5'-phosphate + ADP
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 29000, calculation from nucleotide sequence Drosophila melanogaster

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00257
-
 IC50: 0.00257 mM for reaction with thymidine and 0.00194 mM for reaction with 2'-deoxycytidine Drosophila melanogaster (E)-5-(2-bromovinyl)-2'-deoxyuridine
0.0163
-
 IC50: 0.0163 mM for reaction with thymidine and 0.0154 mM for reaction with 2'-deoxycytidine Drosophila melanogaster 1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-5-iodouracil
0.0258
-
 IC50: 0.0258 mM for reaction with thymidine and 0.02235 mM for reaction with 2'-deoxycytidine Drosophila melanogaster 5-fluoro-2'-deoxyuridine
0.0277
-
 IC50: 0.0277 mM for reaction with thymidine and 0.0158 mM for reaction with 2'-deoxycytidine Drosophila melanogaster (E)-5-(2-bromovinyl)-1-beta-D-arabinofuranosyluracil
0.0419
-
 IC50: 0.0419 mM for reaction with thymidine and 0.0296 mM for reaction with 2'-deoxycytidine Drosophila melanogaster 3'-azido-2',3'-dideoxythymidine
0.0534
-
 IC50: 0.0534 mM for reaction with thymidine and 0.089 mM for reaction with 2'-deoxycytidine Drosophila melanogaster 1-beta-D-arabinofuranosylcytosine
0.057
-
 IC50: 0.057 mM for reaction with thymidine and 0.058 mM for reaction with 2'-deoxycytidine Drosophila melanogaster 1-beta-D-arabinofuranosyluracil
0.0648
-
 IC50: 0.0648 mM for reaction with thymidine and 0.0348 mM for reaction with 2'-deoxycytidine Drosophila melanogaster 1-beta-D-arabinofuranosylthymidine
0.075
-
 IC50: 0.075 mM for reaction with thymidine and 0.0703 mM for reaction with 2'-deoxycytidine Drosophila melanogaster 2',3'-dideoxythymidine
0.123
-
 IC50: 0.123 mM for reaction with thymidine and 0.12 mM for reaction with 2'-deoxycytidine Drosophila melanogaster 2-chloro-2'-deoxyadenosine
0.145
-
 IC50: 0.145 mM for reaction with thymidine and 0.102 mM for reaction with 2'-deoxycytidine Drosophila melanogaster 2',2'-difluorodeoxycytidine
0.74
-
 IC50: 0.74 mM for reaction with thymidine and 0.868 mM for reaction with 2'-deoxycytidine Drosophila melanogaster 2'-deoxy-3'-thiacytidine
0.76
-
 IC50: 0.76 mM for reaction with thymidine and 0.838 mM for reaction with 2'-deoxycytidine Drosophila melanogaster 2',3'-dideoxycytidine
0.805
-
 IC50 for reaction with 2'-deoxycytidine is 0.805 mM Drosophila melanogaster 2',3'-dehydro-3'-deoxythymidine
1
-
 IC50 for reaction with thymidine is greater than 1000 microM Drosophila melanogaster 2',3'-dehydro-3'-deoxythymidine