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Literature summary for 2.7.1.138 extracted from

  • Won, J.H.; Kim, S.K.; Shin, I.C.; Ha, H.C.; Jang, J.M.; Back, M.J.; Kim, D.K.
    Dopamine transporter trafficking is regulated by neutral sphingomyelinase 2/ceramide kinase (2018), Cell. Signal., 44, 171-187 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ceramide kinase inhibitor K1
-
Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + ceramide Mus musculus
-
ADP + ceramide 1-phosphate
-
?
ATP + ceramide Mus musculus C57BL/6
-
ADP + ceramide 1-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus Q8K4Q7
-
-
Mus musculus C57BL/6 Q8K4Q7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Mus musculus
-
corpus striatum
-
Mus musculus
-
PC-12 cell
-
Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ceramide
-
Mus musculus ADP + ceramide 1-phosphate
-
?
ATP + ceramide
-
Mus musculus C57BL/6 ADP + ceramide 1-phosphate
-
?

Synonyms

Synonyms Comment Organism
CERK
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Mus musculus

General Information

General Information Comment Organism
malfunction treatment with ceramide kinase inhibitor K1, or nSMase inhibitor GW4869, decreases dopamine uptake in PC-12 cells, although the application of FB1, a ceramide synthase inhibitor, does not affect dopamine uptake. Transfection of nSMase2 and CERK siRNA decrease the dopamine transporter (DAT) surface level in PC-12 cells. SM-derived ceramide 1-phosphate affects cell surface levels of DAT. Protein-protein interaction between nSMase2 and DAT occur in the mouse striatum. Treatment of CERK knockdown PC-12 cells with C8-ceramide partially rescues the nSMase2 siRNA-induced decrease in DA uptake by about 40%, while treatment of C8-ceramide 1-phosphate also partially rescues the CERK sRNA-induced decrease in DA utake by about 45% Mus musculus
physiological function dopamine transporter trafficking is regulated by neutral sphingomyelinase 2/ceramide kinase (nSMase/CERK). The dopamine transporter (DAT) has an important role in the regulation of Dopamine (DA) reuptake. DAT recycling is regulated by ceramide kinase via the sphingomyelin pathway. Dopamine stimulates the formation of ceramide and increases nSMase activity in PC-12 cells, while treatment with a cell-permeable ceramide 1-phosphate (C1P) increases DA uptake. Endocytic recycling of DAT requires CERK Mus musculus