Cloned (Comment) | Organism |
---|---|
expressed in COS-1 cells | Homo sapiens |
recombinant full-length hCERK is obtained from baculovirus-infected Sf9 cells as a glutathione-S-transferase fusion | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S340A | by site directed mutagenesis, using of Triton X-100 as lysis buffer results in 15% activity decrease in the mutant protein compared with the wild type enzyme, when octylglucoside is used instead of Triton X-100 for cell lysis, activity is reduced in the S340A mutant protein which reaches only 15% of wild type activity | Homo sapiens |
S340D | by site directed mutagenesis, intermediate recovery of 16% is observed for the mutant protein | Homo sapiens |
S427A | by site directed mutagenesis, activity in the S427A mutant protein amounts to only 30% of that of wild type enzyme | Homo sapiens |
S427D | by site directed mutagenesis, mutant protein displays 50% of wild type activity | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ceramide | Homo sapiens | - |
ADP + ceramide 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8TCT0 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation of serine residues at positions 340 and 408, putative additional phosphorylation site at serine 427 lying in the calcium/calmodulin domain, preventing phosphorylation at S340 results in CERK instability | Homo sapiens |
Purification (Comment) | Organism |
---|---|
by affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ceramide | - |
Homo sapiens | ADP + ceramide 1-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ceramide kinase | - |
Homo sapiens |
CERK | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |