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Literature summary for 2.7.1.138 extracted from

  • Don, A.S.; Rosen, H.
    A fluorescent plate reader assay for ceramide kinase (2008), Anal. Biochem., 375, 265-271.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.138

Cloned(Commentary)

Cloned (Comment) Organism
expressed in J-774 cells Homo sapiens
expression in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
dimethylsphingosine DMS Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 N-(6-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)hexanoyl)-D-erythro-sphingosine
-
 Homo sapiens
0.168
-
 ATP
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ceramide + ATP Homo sapiens ceramide kinase is important for cellular proliferation and survival, activation of cytosolic phospholipase A2, mast cell degranulation, and phagocytosis ceramide 1-phosphate + ADP
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
as a C-terminal fusion to maltose binding protein, MBP-Cerk was purified on amylose resin Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-
umbilical vein endothelial cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ceramide + ATP
-
 Homo sapiens ceramide 1-phosphate + ADP
-
 ?
ceramide + ATP ceramide kinase is important for cellular proliferation and survival, activation of cytosolic phospholipase A2, mast cell degranulation, and phagocytosis Homo sapiens ceramide 1-phosphate + ADP
-
 ?
N-(12-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)dodecanoyl)-D-erythro-sphingosine + ATP C12-NBD ceramide Homo sapiens N-(12-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)dodecanoyl)-D-erythro-sphingosine 1-phosphate + ADP
-
 ?
N-(6-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)hexanoyl)-D-erythro-sphingosine + ATP C6-NBD ceramide Homo sapiens N-(6-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)hexanoyl)-D-erythro-sphingosine 1-phosphate + ADP
-
 ?

Synonyms

Synonyms Comment Organism
CERK
-
 Homo sapiens