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Literature summary for 2.7.1.137 extracted from

  • Schoijet, A.C.; Miranda, K.; Girard-Dias, W.; de Souza, W.; Flawia, M.M.; Torres, H.N.; Docampo, R.; Alonso, G.D.
    A Trypanosoma cruzi phosphatidylinositol 3-kinase (TcVps34) is involved in osmoregulation and receptor-mediated endocytosis (2008), J. Biol. Chem., 283, 31541-31550.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, recombinant expression of the His-tagged TcVps34 in Escherichia coli strain BL21(DE3) and complementation of an enzyme-deficient Saccharomyces cerevisiae knockout mutant strain Trypanosoma cruzi

Protein Variants

Protein Variants Comment Organism
additional information in TcVps34-overexpressing cells, the activities of vacuolar-H+-ATPase and vacuolar H+-pyrophosphatase are altered, suggesting defects in the acidification of intracellular compartments, phenotype, overview. Overexpressing cells show resistance to PI3K inhibitors. TcVps34-overexpressing epimastigotes show morphological alterations in the plasma membrane region between the cytostome and the flagellar pocket Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
LY-294,002
-
Trypanosoma cruzi
additional information inhibition of TcVps34 with specific PI3K inhibitors, such as wortmannin and LY294000, result in reduced regulatory volume decrease after hyposmotic stress Trypanosoma cruzi
Wortmannin
-
Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
Trypanosoma cruzi 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol Trypanosoma cruzi TcVps34 specifically phosphorylates phosphatidylinositol to produce phosphatidylinositol 3-phosphate ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
?
ATP + 1-phosphatidyl-1D-myo-inositol Trypanosoma cruzi CL Brener TcVps34 specifically phosphorylates phosphatidylinositol to produce phosphatidylinositol 3-phosphate ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi A9QWR8 CL Brener strain
-
Trypanosoma cruzi CL Brener A9QWR8 CL Brener strain
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged TcVps34 from Escherichia coli strain BL21(DE3) by affinity chromatography Trypanosoma cruzi

Source Tissue

Source Tissue Comment Organism Textmining
epimastigote
-
Trypanosoma cruzi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol TcVps34 specifically phosphorylates phosphatidylinositol to produce phosphatidylinositol 3-phosphate Trypanosoma cruzi ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
?
ATP + 1-phosphatidyl-1D-myo-inositol TcVps34 specifically phosphorylates phosphatidylinositol to produce phosphatidylinositol 3-phosphate Trypanosoma cruzi CL Brener ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
?

Synonyms

Synonyms Comment Organism
More TcVps34 belongs to the class III PI3K family Trypanosoma cruzi
PI3K
-
Trypanosoma cruzi
TcVps34
-
Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
ATP
-
Trypanosoma cruzi

General Information

General Information Comment Organism
malfunction inhibition of TcVps34 with specific PI3K inhibitors, such as wortmannin and LY294000, result in reduced regulatory volume decrease after hyposmotic stress Trypanosoma cruzi
physiological function the Trypanosoma cruzi phosphatidylinositol 3-kinase, TcVps34, is involved in osmoregulation and receptor-mediated endocytosis. TcVps34 participates in the endocytic pathway, fluid phase uptake of BSA and receptor-mediated endocytosis of transferrin. Multiple roles for TcVps34, schematic overview Trypanosoma cruzi