Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, recombinant expression of the His-tagged TcVps34 in Escherichia coli strain BL21(DE3) and complementation of an enzyme-deficient Saccharomyces cerevisiae knockout mutant strain | Trypanosoma cruzi |
Protein Variants | Comment | Organism |
---|---|---|
additional information | in TcVps34-overexpressing cells, the activities of vacuolar-H+-ATPase and vacuolar H+-pyrophosphatase are altered, suggesting defects in the acidification of intracellular compartments, phenotype, overview. Overexpressing cells show resistance to PI3K inhibitors. TcVps34-overexpressing epimastigotes show morphological alterations in the plasma membrane region between the cytostome and the flagellar pocket | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
LY-294,002 | - |
Trypanosoma cruzi | |
additional information | inhibition of TcVps34 with specific PI3K inhibitors, such as wortmannin and LY294000, result in reduced regulatory volume decrease after hyposmotic stress | Trypanosoma cruzi | |
Wortmannin | - |
Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Trypanosoma cruzi | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol | Trypanosoma cruzi | TcVps34 specifically phosphorylates phosphatidylinositol to produce phosphatidylinositol 3-phosphate | ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol | Trypanosoma cruzi CL Brener | TcVps34 specifically phosphorylates phosphatidylinositol to produce phosphatidylinositol 3-phosphate | ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | A9QWR8 | CL Brener strain | - |
Trypanosoma cruzi CL Brener | A9QWR8 | CL Brener strain | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged TcVps34 from Escherichia coli strain BL21(DE3) by affinity chromatography | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epimastigote | - |
Trypanosoma cruzi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol | TcVps34 specifically phosphorylates phosphatidylinositol to produce phosphatidylinositol 3-phosphate | Trypanosoma cruzi | ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol | TcVps34 specifically phosphorylates phosphatidylinositol to produce phosphatidylinositol 3-phosphate | Trypanosoma cruzi CL Brener | ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | TcVps34 belongs to the class III PI3K family | Trypanosoma cruzi |
PI3K | - |
Trypanosoma cruzi |
TcVps34 | - |
Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Trypanosoma cruzi |
General Information | Comment | Organism |
---|---|---|
malfunction | inhibition of TcVps34 with specific PI3K inhibitors, such as wortmannin and LY294000, result in reduced regulatory volume decrease after hyposmotic stress | Trypanosoma cruzi |
physiological function | the Trypanosoma cruzi phosphatidylinositol 3-kinase, TcVps34, is involved in osmoregulation and receptor-mediated endocytosis. TcVps34 participates in the endocytic pathway, fluid phase uptake of BSA and receptor-mediated endocytosis of transferrin. Multiple roles for TcVps34, schematic overview | Trypanosoma cruzi |