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Literature summary for 2.7.1.136 extracted from
- Nucleotide selectivity of antibiotic kinases (2010), Antimicrob. Agents Chemother., 54, 1909-1913.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | competitive assay that mimics in vivo nucleotide triphosphate concentrations and usage by the enzyme. Downstream analysis of reaction products by high-performance liquid chromatography enables the determination of partitioning of phosphate flux from nucleotide triphosphate donors to antibiotics | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Adenylyl imidodiphosphate | - |
Escherichia coli |  |
| additional information | not inhibitory: guanylyl imidodiphosphate | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0106 | - |
ATP | pH 7.5, 37°C | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | under physiological conditions, enzyme exclusively uses ATP, no substrate: GTP | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | Q47396 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + azithromycin | - |
Escherichia coli | ADP + azithromycin 2'-O-phosphate | - |
? | |
| ATP + clarithromycin | - |
Escherichia coli | ADP + clarithromycin 2'-O-phosphate | - |
? | |
| ATP + erythromycin | - |
Escherichia coli | ADP + erythromycin 2'-O-phosphate | - |
? | |
| ATP + neomycin | - |
Escherichia coli | ADP + ? | - |
? | |
| ATP + spiramycin | - |
Escherichia coli | ADP + spiramycin 2'-O-phosphate | - |
? | |
| ATP + telithromycin | - |
Escherichia coli | ADP + telithromycin 2'-O-phosphate | - |
? | |
| ATP + tylosin | - |
Escherichia coli | ADP + tylosin 2'-phosphate | - |
? | |
| additional information | under physiological conditions, enzyme exclusively uses ATP, no substrate: GTP | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| macrolide 2'-phosphotransferase | - |
Escherichia coli |
| mphA | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.59 | - |
ATP | pH 7.5, 37°C | Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0142 | - |
Adenylyl imidodiphosphate | pH 7.5, 37°C | Escherichia coli | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 150 | - |
ATP | pH 7.5, 37°C | Escherichia coli | |
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