Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha, complete pRSB105 macrolide resistance gene region cloned into the vector pBCSK, recombinant plasmid pNK-mph carries the complete mph gene in pBluescript II KS, transcription from the constructed plasmids analyzed by RT-PCR, constructs tested for conferring erythromycin and azithromycin resistance | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | Q47396 | recombinant, carrying plasmid pRSB105 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
genetic map of the resistance plasmid pRSB105, functional antibiotic resistance regions of plasmid pRSB105 characterized, plasmid pRSB105 harbors the macrolide resistance gene mph, encoding a macrolide 2'-phosphotransferase, transcript analysis by RT-PCR, erythromycin resistance analyzed by determining values for minimum inhibitory concentration, mph gene shown to contribute to erythromycin resistance | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + erythromycin | erythromycin resistance analyzed in recombinant Escherichia coli harbouring plasmid pRSB105 | Escherichia coli | ADP + erythromycin 2'-O-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
macrolide 2'-phosphotransferase | - |
Escherichia coli |