Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.136 extracted from

  • Schlueter, A.; Szczepanowski, R.; Kurz, N.; Schneiker, S.; Krahn, I.; Puehler, A.
    Erythromycin resistance-conferring plasmid pRSB105, isolated from a sewage treatment plant, harbors a new macrolide resistance determinant, an integron-containing Tn402-like element, and a large region of unknown function (2007), Appl. Environ. Microbiol., 73, 1952-1960.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha, complete pRSB105 macrolide resistance gene region cloned into the vector pBCSK, recombinant plasmid pNK-mph carries the complete mph gene in pBluescript II KS, transcription from the constructed plasmids analyzed by RT-PCR, constructs tested for conferring erythromycin and azithromycin resistance Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli Q47396 recombinant, carrying plasmid pRSB105
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
genetic map of the resistance plasmid pRSB105, functional antibiotic resistance regions of plasmid pRSB105 characterized, plasmid pRSB105 harbors the macrolide resistance gene mph, encoding a macrolide 2'-phosphotransferase, transcript analysis by RT-PCR, erythromycin resistance analyzed by determining values for minimum inhibitory concentration, mph gene shown to contribute to erythromycin resistance Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + erythromycin erythromycin resistance analyzed in recombinant Escherichia coli harbouring plasmid pRSB105 Escherichia coli ADP + erythromycin 2'-O-phosphate
-
?

Synonyms

Synonyms Comment Organism
macrolide 2'-phosphotransferase
-
Escherichia coli