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Literature summary for 2.7.1.136 extracted from

  • O'Hara, K.; Kanda,T.; Ohmiya, K.; Ebisu, T.; Kono, M.
    Purification and characterization of macrolide 2'-phosphotransferase from a strain of Escherichia coli that is highly resistant to erythromycin (1989), Antimicrob. Agents Chemother., 33, 1354-1357.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
erythromycin crude cell extracts from cells treated with erythromycin show increased activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
CoSO4 1 mM, 51% inhibition Escherichia coli
CuSO4 1 mM, 52% inhibition Escherichia coli
EDTA 10 mM, 100% inhibition Escherichia coli
FeSO4 1 mM, 66% inhibition Escherichia coli
Iodine 1 mM, 100% inhibition Escherichia coli
additional information 1 mM p-chloromercuribenzoic acid or urea are no inhibitors, MphR(A) is a repressor protein that binds to the promoter of the mph(A) gene and negatively regulates the enzyme expression Escherichia coli
ZnSO4 1 mM, 93% inhibition Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Escherichia coli 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ essential Escherichia coli
additional information potassium chloride enhances reactivity, it is added in the reaction mixture at 0.06 M Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
x * 34000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli Q47396 macrolide 2'-phosphotransferase type I, high levels of activity with 14-member ring macrolides and extremely low levels with 16-member ring macrolides, accession code corresponds to nucleotide sequence for several proteins
-

Storage Stability

Storage Stability Organism
-20°C, Tris-HCl buffer pH 7, 0.06 M KCl, 0.01 M MgCl2, 0.006 M 2-mercaptoethanol, stable Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + erythromycin 87% activity with respect to oleandomycin Escherichia coli ADP + erythromycin-2'-O-phosphate
-
?
ATP + josamycin 1% activity with respect to oleandomycin Escherichia coli ?
-
?
ATP + midecamycin 3% activity with respect to oleandomycin Escherichia coli ?
-
?
ATP + oleandomycin
-
Escherichia coli ADP + oleandomycin 2'-O-phosphate
-
?
ATP + spectinomycin 5% activity with respect to oleandomycin Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Escherichia coli