Cloned (Comment) | Organism |
---|---|
stable expression of the C-terminal catalytic domain 3KA-cat30, comprising residues Ser185-Arg459, as N-terminally His-tagged and S-tagged protein in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2'S)-1D-1,2-O-[(2'-phosphoryloxy)propane-1',3'-diyl]-myo-inositol 4,5-bisphosphate | synthetic bicyclic inositol trisphosphate S epimer, IC50 is 0.156 mM | Rattus norvegicus | |
1D-myo-inositol 1,3,4,5-tetrakisphosphate | product inhibition, IC50 is 0.013 mM | Rattus norvegicus | |
D-2-deoxyinositol 1,3,4,5-tetrakisphosphate | strong inhibition of isozyme A, IC50 is 0.0054 mM | Rattus norvegicus | |
D-2-deoxyinositol 1,4,5-trisphosphate | strong inhibition of isozyme A, IC50 is 0.0017 mM | Rattus norvegicus | |
D-3-deoxyinositol 1,4,6-trisphosphate | strong inhibition of isozyme A, IC50 is 0.0014 mM | Rattus norvegicus | |
D-6-deoxyinositol 1,3,4,5-tetrakisphosphate | strong inhibition of isozyme A, IC50 is 0.0051 mM | Rattus norvegicus | |
D-myo-inositol 2,4,5-trisphosphate | IC50 is 0.117 mM | Rattus norvegicus | |
additional information | poor inhibition by adenophostin analogues xylo-furanophostin and furanophostin, by L-myo-inositol 2,4,5-trisphosphate, synthetic bicyclic inositol trisphosphate R epimer, and by epi-1D-myo-inositol 1,3,6-trisphosphate | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus | |
additional information | assay in presence of Triton X-100 and ethylene glycol bis-(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Rattus norvegicus | - |
ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
isozyme A | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged and S-tagged C-terminal catalytic domain 3KA-cat30, comprising residues Ser185-Arg459, from Escherichia coli strain BL21(DE3) by nickel and S-protein affinity chromatography, and gel filtration | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate = ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | catalytic mechanism involving the 3-hydroxygroup of the substrate, substrate recognition | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + D-2-deoxy-myo-inositol 1,4,5-trisphosphate | - |
Rattus norvegicus | ADP + D-2-deoxy-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
inositol 1,4,5-trisphosphate 3-kinase A | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0014 | - |
strong inhibition of isozyme A, IC50 is 0.0014 mM | Rattus norvegicus | D-3-deoxyinositol 1,4,6-trisphosphate | |
0.0017 | - |
strong inhibition of isozyme A, IC50 is 0.0017 mM | Rattus norvegicus | D-2-deoxyinositol 1,4,5-trisphosphate | |
0.0051 | - |
strong inhibition of isozyme A, IC50 is 0.0051 mM | Rattus norvegicus | D-6-deoxyinositol 1,3,4,5-tetrakisphosphate | |
0.0054 | - |
strong inhibition of isozyme A, IC50 is 0.0054 mM | Rattus norvegicus | D-2-deoxyinositol 1,3,4,5-tetrakisphosphate | |
0.013 | - |
product inhibition, IC50 is 0.013 mM | Rattus norvegicus | 1D-myo-inositol 1,3,4,5-tetrakisphosphate | |
0.117 | - |
IC50 is 0.117 mM | Rattus norvegicus | D-myo-inositol 2,4,5-trisphosphate | |
0.156 | - |
synthetic bicyclic inositol trisphosphate S epimer, IC50 is 0.156 mM | Rattus norvegicus | (2'S)-1D-1,2-O-[(2'-phosphoryloxy)propane-1',3'-diyl]-myo-inositol 4,5-bisphosphate |