Activating Compound | Comment | Organism | Structure |
---|---|---|---|
protein kinase A | isoforms A and B are differentially regulated via phosphorylation by the cAMP-dependent protein kinase, isoform A: phosphorylated to the extent of 0.9 mol/mol, 2.5fold stimulation in the absence of Ca2+/calmodulin, 1.5fold stimulation in the presence of Ca2+/calmodulin, isoform B: phosphorylated to the extent of 1 mol/mol, no effect on activity in the absence of Ca2+/calmodulin, 45% inhibition in the presence of Ca2+/calmodulin | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
cDNAs encoding enzyme isoforms A from brain and B are cloned and overexpressed in B31 cells, a rat-1 fibroblast cell line expressing pp60v-src | Rattus norvegicus |
General Stability | Organism |
---|---|
2-mercaptoethanol and Triton X-100 or CHAPS stabilize during purification | Rattus norvegicus |
bovine serum albumin stabilizes dilute enzyme solutions | Rattus norvegicus |
isoform A is more susceptible to proteolysis during purification than isoform B | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | isoforms A and B, above 0.01 mM | Rattus norvegicus | |
protein kinase A | isoforms A and B are differentially regulated via phosphorylation by the cAMP-dependent protein kinase, isoform A: stimulation in the presence or absence of Ca2+/calmodulin, isoform B: no effect on activity in the absence of Ca2+/calmodulin, 45% inhibition in the presence of Ca2+/calmodulin | Rattus norvegicus | |
Protein kinase C | isoforms A and B are differentially regulated via phosphorylation by protein kinase C, isoform A: phosphorylated to the extent of 2 mol/mol, 72% inhibition in the absence of Ca2+/calmodulin, isoform B: phosphorylated to the extent of 2.7 mol/mol, no effect on activity in the absence of Ca2+/calmodulin, both isoforms are inhibited by 70% in the presence of Ca2+/calmodulin | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | 0.005 | 1D-myo-inositol 1,4,5-trisphosphate | pH 7.5, 37°C, recombinant isoenzymes A and B | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | isoforms A and B are differentially regulated by Ca2+/calmodulin, isoform A: 2.5fold stimulation, isoform B: 20fold stimulation | Rattus norvegicus | |
Ca2+ | activation by Ca2+/calmodulin | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
x * 59000, recombinant isoform A, x * 92000, recombinant isoform B, SDS-PAGE | Rattus norvegicus |
92000 | - |
x * 59000, recombinant isoform A, x * 92000, recombinant isoform B, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Rattus norvegicus | differential expression and regulation of the two enzyme isoforms A and B provides multiple mechanisms for regulating the cytosolic level of inositol 1,4,5-trisphosphate in cells, regulation mechanisms | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Rattus norvegicus | inositol 1,4,5-trisphosphate is a second messenger | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Triton X-100 | stabilizes | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
2 isoenzymes: A and B | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | isoforms A and B are differentially regulated via phosphorylation by protein kinase C and the cAMP-dependent protein kinase, potential phosphorylation sites | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
recombinant isoforms A and B, overexpressed in B31 rat fibroblast, isoform A: 11005fold, isoform B: 43806fold | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | isoform A | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.77 | - |
pH 7.5, 37°C, recombinant isoform B | Rattus norvegicus |
36.32 | - |
pH 7.5, 37°C, recombinant isoform A | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-70°C, buffer containing detergent and reducing agent, pH 7-8, at least 3 months, stable | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | multiple regulation mechanisms of enzyme isoform A and B | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | differential expression and regulation of the two enzyme isoforms A and B provides multiple mechanisms for regulating the cytosolic level of inositol 1,4,5-trisphosphate in cells, regulation mechanisms | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | inositol 1,4,5-trisphosphate is a second messenger | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59000, recombinant isoform A, x * 92000, recombinant isoform B, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus | |
Calmodulin | activation by Ca2+/calmodulin | Rattus norvegicus | |
Calmodulin | isoforms A and B are differentially regulated by Ca2+/calmodulin, isoform A: 2.5fold stimulation, isoform B: 20fold stimulation | Rattus norvegicus |