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Literature summary for 2.7.1.127 extracted from

  • Wang, X.L.; Akhtar, R.A.; Abdel-Latif, A.A.
    Purification and properties of D-myo-inositol 1,4,5-trisphosphate 3-kinase from bovine iris sphincter smooth muscle: effects of protein phosphorylation in vitro and in intact muscle (1995), Biochem. J., 308, 1009-1016.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.127

Activating Compound

Activating Compound Comment Organism Structure
isoproterenol activates Bos taurus
phorbol 12,13-dibutyrate activates Bos taurus
protein kinase A phosphorylates and activates IP3K Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ at concentrations higher than 0.01-0.1 mM Bos taurus
Protein kinase C phosphorylates and inactivates enzyme Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00056
-
 1D-myo-inositol 1,4,5-trisphosphate pH 7.2, 37°C, in presence or absence of 50 nM calmodulin Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble predominantly Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ maximal at 0.01-0.1 mM Ca2+, interaction between the Ca2+-calmodulin complex and enzyme Bos taurus
Ca2+ activation by Ca2+/calmodulin Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate Bos taurus role in limiting the availability of InsP3 for Ca2+ release in smooth muscle relaxation ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,4,5-trisphosphate Bos taurus inositol phosphate metabolism ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
114fold Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
iris sphincter muscle
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.94
-
 pH 7.2, 37°C Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 1,4,5-trisphosphate specific for phosphorylation of the 3-position Bos taurus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,4,5-trisphosphate role in limiting the availability of InsP3 for Ca2+ release in smooth muscle relaxation Bos taurus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,4,5-trisphosphate inositol phosphate metabolism Bos taurus ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Bos taurus
7.2
-
 assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Bos taurus
Calmodulin activation by Ca2+/calmodulin Bos taurus
Calmodulin maximal at 50 nM calmodulin, reversed by EGTA, interaction between the Ca2+-calmodulin complex and enzyme Bos taurus