Cloned (Comment) | Organism |
---|---|
entire gene encoding IP3K is cloned and 130fold overexpressed in Escherichia coli BL21(DE3), when induced by isopropyl-beta-D-thiogalactoside | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
1D-myo-inositol 1,4,5-trisphosphate | recombinant IP3K, in absence of Ca2+/calmodulin, 37°C | Rattus norvegicus | |
0.0876 | - |
ATP | 37°C, recombinant IP3K | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | mainly | Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1.5fold activation by Ca2+/calmodulin | Rattus norvegicus | |
Ca2+ | activation by Ca2+/calmodulin | Rattus norvegicus | |
Ca2+ | not activated by free Ca2+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, recombinant IP3K overexpressed in Escherichia coli, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Rattus norvegicus | IP3K regulates the intracellular level of the 2 second messengers Ins(1,4,5)P3 and Ins(1,3,4,5)P4 | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Rattus norvegicus | inositol phosphate metabolism | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
83fold | Rattus norvegicus |
recombinant IP3K overexpressed in Escherichia coli | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20 | - |
recombinant IP3K overexpressed in Escherichia coli, in absence of calmodulin | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | IP3K regulates the intracellular level of the 2 second messengers Ins(1,4,5)P3 and Ins(1,3,4,5)P4 | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | inositol phosphate metabolism | Rattus norvegicus | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, recombinant IP3K overexpressed in Escherichia coli, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus | |
Calmodulin | activation by Ca2+/calmodulin | Rattus norvegicus | |
Calmodulin | 1.5fold activation by Ca2+/calmodulin | Rattus norvegicus |