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Literature summary for 2.7.1.12 extracted from

  • Leyva, L.A.; Bashan, Y.
    Activity of two catabolic enzymes of the phosphogluconate pathway in mesquite roots inoculated with Azospirillum brasilense Cd (2008), Plant Physiol. Biochem., 46, 898-904.
    View publication on PubMed
Search for more literature for 2.7.1.12

Activating Compound

Activating Compound Comment Organism Structure
gluconic acid gluconokinase is induced upon exposure to gluconic acid Azospirillum brasilense

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 10 mM are included in assay medium Azospirillum brasilense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-gluconate + ATP Azospirillum brasilense
-
 6-phospho-D-gluconate + ADP
-
 ?
D-gluconate + ATP Azospirillum brasilense Cd
-
 6-phospho-D-gluconate + ADP
-
 ?

Organism

Organism UniProt Comment Textmining
Azospirillum brasilense
-
 Cd
-
Azospirillum brasilense Cd
-
 Cd
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 at six concentrations (4.6-90.6 mM) of gluconate added to culture medium for Azospirillum brasilense Cd grown in TYG medium, activity of gluconokinase activity is positively affected Azospirillum brasilense
additional information
-
 gluconokinase activity significantly increases in inoculated mesquite plants incubated for 23 days, inoculation is not enhanced after 15 days of incubation Azospirillum brasilense
additional information
-
 while increased gluconokinase activity results only in inoculated treatments, older non-inoculated seedlings maintained the same activity as younger seedlings and the sum of activities of the roots and the negative control is still lower than activity in inoculated plants Azospirillum brasilense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-gluconate + ATP
-
 Azospirillum brasilense 6-phospho-D-gluconate + ADP
-
 ?
D-gluconate + ATP
-
 Azospirillum brasilense Cd 6-phospho-D-gluconate + ADP
-
 ?

Synonyms

Synonyms Comment Organism
gluconokinase
-
 Azospirillum brasilense

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Azospirillum brasilense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Azospirillum brasilense

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Azospirillum brasilense

Expression

Organism Comment Expression
Azospirillum brasilense glucokinase is induced upon exposure to gluconic acid up

General Information

General Information Comment Organism
metabolism phosphogluconate pathway, bacterial enzyme is active in young, non-inoculated mesquite seedlings growing under hydroponic conditions Azospirillum brasilense
physiological function when azospirillum brasilense Cd bacteria are inoculated on the root system, the roots exhibit much higher activity of gluconokinase Azospirillum brasilense