Activating Compound | Comment | Organism | Structure |
---|---|---|---|
gluconate | 6-phosphogluconate dehydrogenase, although constituvely expressed, shows a 3fold higher specific level in gluconate grown cells than those grown in fructose under similar conditions | Corynebacterium glutamicum |
Cloned (Comment) | Organism |
---|---|
in Escherichia coli | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
predicted from 167 amino acids of gluconate kinase | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate + ATP | Corynebacterium glutamicum | - |
6-phospho-D-gluconate + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | Q8NMT0 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Corynebacterium glutamicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.047 | - |
activity of glucokinase in Corynebacterium glutamicum (ATCC 13032) culture in MCGC medium at 37°C, supplemented with 0.5% gluconate | Corynebacterium glutamicum |
0.388 | - |
activity of glucokinase expressed in Escherichia strain M1-8, supplemented with fructose as carbon source | Corynebacterium glutamicum |
1.429 | - |
transformed Escherichia coli cells (strain DH5alpha carrying the pTAEK plasmid), grown in Lb and collected during the exponential phase, then cultivated in CAA medium with 0.2% fructose as carbon source | Corynebacterium glutamicum |
2.894 | - |
transformed Escherichia coli cells (mutant TGN282 carrying the pTAEK plasmid), grown in Lb and collected during the exponential phase, then cultivated in CAA medium with 0.2% fructose as carbon source | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate + ATP | - |
Corynebacterium glutamicum | 6-phospho-D-gluconate + ADP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gluconokinase | - |
Corynebacterium glutamicum |
gntK | - |
Corynebacterium glutamicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Corynebacterium glutamicum |
General Information | Comment | Organism |
---|---|---|
metabolism | pentose phosphate pathway | Corynebacterium glutamicum |