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Literature summary for 2.7.1.12 extracted from
- Purification and characterization of the Escherichia coli thermoresistant glucokinase encoded by the gntK gene (1996), FEBS Lett., 394, 14-16.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
D-gluconate | - |
Escherichia coli |  |
| 0.042 | - |
D-gluconate | 25°C, pH 7 | Escherichia coli | |
| 0.045 | - |
ATP | - |
Escherichia coli | |
| 0.123 | - |
ATP | 25°C, pH 7 | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18400 | - |
2 * 18400, SDS-PAGE | Escherichia coli |
| 29500 | - |
alpha3, 3 * 29500 | Escherichia coli |
| 38000 | - |
gel filtration | Escherichia coli |
| 100000 | - |
- |
Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-gluconate | Escherichia coli | - |
ADP + 6-phospho-D-gluconate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K-12 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Escherichia coli |
| 250 | - |
thermoresistant gluconokinase encoded by th gntK | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-gluconate | - |
Escherichia coli | ADP + 6-phospho-D-gluconate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 18400, SDS-PAGE | Escherichia coli |
| trimer | alpha3, 3 * 29500 | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
thermoresistant gluconokinase encoded by th gntK | Escherichia coli |
| 55 | - |
- |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
after 40 min, 50% loss of activity | Escherichia coli |
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