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Literature summary for 2.7.1.12 extracted from

  • Leder, I.G.
    Hog kidney gluconokinase (1957), J. Biol. Chem., 225, 125-136.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ ATP protects Sus scrofa
Mg2+ at each level of ATP the optimal molar concentration of Mg2+ is one-half that of ATP, higher concentration inhibits Sus scrofa
additional information not: iodoacetate Sus scrofa
NaF not Sus scrofa
p-chloromercuribenzoate
-
Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can partially replace Mg2+ in activation Sus scrofa
Co2+ can partially replace Mg2+ in activation Sus scrofa
Mg2+ activates Sus scrofa
Mg2+ at each level of ATP the optimal molar concentration of Mg2+ is one-half that of ATP, higher concentration inhibits Sus scrofa
Mn2+ can partially replace Mg2+ in activation Sus scrofa
Zn2+ can partially replace Mg2+ in activation Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-gluconate Sus scrofa
-
ADP + 6-phospho-D-gluconate
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.64
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
-15°C, 10% loss of activity after 3 weeks Sus scrofa
3°C, 50% loss of activity after 3 weeks Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-gluconate
-
Sus scrofa ADP + 6-phospho-D-gluconate
-
?
ATP + D-gluconate strict specificity for D-gluconate Sus scrofa ADP + 6-phospho-D-gluconate
-
?
GTP + D-gluconate 25% of the activity with ATP Sus scrofa GDP + 6-phospho-D-gluconate
-
?
ITP + D-gluconate 60% of the activity with ATP Sus scrofa IDP + 6-phospho-D-gluconate
-
?
UTP + D-gluconate 130% of the activity with ATP Sus scrofa UDP + 6-phospho-D-gluconate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
34
-
assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
5.1 8.4 pH 5.1: about 25% of activity maximum, pH 8.4: about 50% of activity maximum Sus scrofa