BRENDA - Enzyme Database
show all sequences of 2.7.1.114

Thymidine phosphotransferase and nucleotide phosphohydrolase of the fern Asplenium nidus. General properties and inhibition by adenosine 3:5-cyclic monophosphate

Grivell, A.R.; Jackson, J.F.; Biochem. J. 155, 571-581 (1976)

Data extracted from this reference:

General Stability
General Stability
Organism
bovine serum albumin stabilizes
Asplenium nidus
not stabilized by AMP or thymidine
Asplenium nidus
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Asplenium nidus
3',5'-cAMP
-
Asplenium nidus
adenosine
-
Asplenium nidus
CoCl2
-
Asplenium nidus
MnCl2
-
Asplenium nidus
NiCl2
-
Asplenium nidus
uridine
-
Asplenium nidus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
AMP
38°C, pH 7.5
Asplenium nidus
0.84
0.88
thymidine
38°C, pH 7.5
Asplenium nidus
1.28
-
adenosine
38°C, pH 7.5
Asplenium nidus
4.71
-
uridine
38°C, pH 7.5
Asplenium nidus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
AMP + thymidine
Hordeum vulgare
-
adenosine + dTMP
-
-
?
AMP + thymidine
Medicago sativa
-
adenosine + dTMP
-
-
?
AMP + thymidine
Helianthus tuberosus
-
adenosine + dTMP
-
-
?
AMP + thymidine
Asplenium nidus
-
adenosine + dTMP
-
-
?
AMP + thymidine
Epidendrum hybrid
-
adenosine + dTMP
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Asplenium nidus
-
bird's nest fern
-
Epidendrum hybrid
-
crucifix orchid
-
Helianthus tuberosus
-
Jerusalem artichoke
-
Hordeum vulgare
-
barley
-
Medicago sativa
-
lucerne
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Asplenium nidus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Asplenium nidus
-
leaf
-
Epidendrum hybrid
-
leaf
-
Helianthus tuberosus
-
pollen
-
Medicago sativa
-
root
-
Hordeum vulgare
-
stem
-
Helianthus tuberosus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.002
-
leaf
Medicago sativa
0.01
-
stem
Helianthus tuberosus
0.05
-
leaf
Epidendrum hybrid
0.05
-
roots
Hordeum vulgare
0.06
-
tops
Hordeum vulgare
0.07
-
senescent leaf
Asplenium nidus
0.29
-
mature leaf
Helianthus tuberosus
0.47
-
young and expanded leaf
Helianthus tuberosus
0.84
-
mature leaf
Asplenium nidus
0.91
-
young mature leaf
Asplenium nidus
1.09
-
immature leaf
Asplenium nidus
1.64
-
young bud
Asplenium nidus
Storage Stability
Storage Stability
Organism
0°C, 6 months, 20% loss of activity
Asplenium nidus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
AMP + 5-bromodeoxyuridine
-
640570
Asplenium nidus
adenosine + 5-bromodeoxyuridine 5'-phosphate
-
-
-
?
AMP + adenosine
-
640570
Asplenium nidus
adenosine + AMP
-
-
-
?
AMP + cytidine
-
640570
Asplenium nidus
adenosine + cytidine 5'-phosphate
-
-
-
?
AMP + deoxyuridine
-
640570
Asplenium nidus
adenosine + deoxyuridine 5'-phosphate
-
-
-
?
AMP + guanosine
-
640570
Asplenium nidus
adenosine + guanosine 5'-phosphate
-
-
-
?
AMP + thymidine
-
640570
Hordeum vulgare
adenosine + dTMP
-
-
-
?
AMP + thymidine
-
640570
Hordeum vulgare
adenosine + dTMP
-
640570
Hordeum vulgare
?
AMP + thymidine
-
640570
Medicago sativa
adenosine + dTMP
-
-
-
?
AMP + thymidine
-
640570
Medicago sativa
adenosine + dTMP
-
640570
Medicago sativa
?
AMP + thymidine
-
640570
Helianthus tuberosus
adenosine + dTMP
-
-
-
?
AMP + thymidine
-
640570
Helianthus tuberosus
adenosine + dTMP
-
640570
Helianthus tuberosus
?
AMP + thymidine
-
640570
Asplenium nidus
adenosine + dTMP
-
-
-
?
AMP + thymidine
-
640570
Epidendrum hybrid
adenosine + dTMP
-
-
-
?
AMP + thymidine
-
640570
Epidendrum hybrid
adenosine + dTMP
-
640570
Epidendrum hybrid
?
AMP + thymidine
AMP can be substituted by GMP, dAMP, CMP, UMP, dUMP, dTMP
640570
Asplenium nidus
adenosine + dTMP
-
640570
Asplenium nidus
?
AMP + uridine
-
640570
Asplenium nidus
adenosine + uridine 5'-phosphate
-
-
-
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
60 min, 95% loss of activity
Asplenium nidus
63
-
5 min, complete inactivation
Asplenium nidus
General Stability (protein specific)
General Stability
Organism
bovine serum albumin stabilizes
Asplenium nidus
not stabilized by AMP or thymidine
Asplenium nidus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Asplenium nidus
3',5'-cAMP
-
Asplenium nidus
adenosine
-
Asplenium nidus
CoCl2
-
Asplenium nidus
MnCl2
-
Asplenium nidus
NiCl2
-
Asplenium nidus
uridine
-
Asplenium nidus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.11
-
AMP
38°C, pH 7.5
Asplenium nidus
0.84
0.88
thymidine
38°C, pH 7.5
Asplenium nidus
1.28
-
adenosine
38°C, pH 7.5
Asplenium nidus
4.71
-
uridine
38°C, pH 7.5
Asplenium nidus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
AMP + thymidine
Hordeum vulgare
-
adenosine + dTMP
-
-
?
AMP + thymidine
Medicago sativa
-
adenosine + dTMP
-
-
?
AMP + thymidine
Helianthus tuberosus
-
adenosine + dTMP
-
-
?
AMP + thymidine
Asplenium nidus
-
adenosine + dTMP
-
-
?
AMP + thymidine
Epidendrum hybrid
-
adenosine + dTMP
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Asplenium nidus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Asplenium nidus
-
leaf
-
Epidendrum hybrid
-
leaf
-
Helianthus tuberosus
-
pollen
-
Medicago sativa
-
root
-
Hordeum vulgare
-
stem
-
Helianthus tuberosus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.002
-
leaf
Medicago sativa
0.01
-
stem
Helianthus tuberosus
0.05
-
leaf
Epidendrum hybrid
0.05
-
roots
Hordeum vulgare
0.06
-
tops
Hordeum vulgare
0.07
-
senescent leaf
Asplenium nidus
0.29
-
mature leaf
Helianthus tuberosus
0.47
-
young and expanded leaf
Helianthus tuberosus
0.84
-
mature leaf
Asplenium nidus
0.91
-
young mature leaf
Asplenium nidus
1.09
-
immature leaf
Asplenium nidus
1.64
-
young bud
Asplenium nidus
Storage Stability (protein specific)
Storage Stability
Organism
0°C, 6 months, 20% loss of activity
Asplenium nidus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
AMP + 5-bromodeoxyuridine
-
640570
Asplenium nidus
adenosine + 5-bromodeoxyuridine 5'-phosphate
-
-
-
?
AMP + adenosine
-
640570
Asplenium nidus
adenosine + AMP
-
-
-
?
AMP + cytidine
-
640570
Asplenium nidus
adenosine + cytidine 5'-phosphate
-
-
-
?
AMP + deoxyuridine
-
640570
Asplenium nidus
adenosine + deoxyuridine 5'-phosphate
-
-
-
?
AMP + guanosine
-
640570
Asplenium nidus
adenosine + guanosine 5'-phosphate
-
-
-
?
AMP + thymidine
-
640570
Hordeum vulgare
adenosine + dTMP
-
-
-
?
AMP + thymidine
-
640570
Hordeum vulgare
adenosine + dTMP
-
640570
Hordeum vulgare
?
AMP + thymidine
-
640570
Medicago sativa
adenosine + dTMP
-
-
-
?
AMP + thymidine
-
640570
Medicago sativa
adenosine + dTMP
-
640570
Medicago sativa
?
AMP + thymidine
-
640570
Helianthus tuberosus
adenosine + dTMP
-
-
-
?
AMP + thymidine
-
640570
Helianthus tuberosus
adenosine + dTMP
-
640570
Helianthus tuberosus
?
AMP + thymidine
-
640570
Asplenium nidus
adenosine + dTMP
-
-
-
?
AMP + thymidine
-
640570
Epidendrum hybrid
adenosine + dTMP
-
-
-
?
AMP + thymidine
-
640570
Epidendrum hybrid
adenosine + dTMP
-
640570
Epidendrum hybrid
?
AMP + thymidine
AMP can be substituted by GMP, dAMP, CMP, UMP, dUMP, dTMP
640570
Asplenium nidus
adenosine + dTMP
-
640570
Asplenium nidus
?
AMP + uridine
-
640570
Asplenium nidus
adenosine + uridine 5'-phosphate
-
-
-
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
37
-
60 min, 95% loss of activity
Asplenium nidus
63
-
5 min, complete inactivation
Asplenium nidus
Other publictions for EC 2.7.1.114
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
640571
Labenz
Inhibition of the Herpes simpl ...
Herpes simplex virus
Arch. Virol.
81
205-212
1984
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
640569
Falke
Adenylic acid: deoxythymidine ...
Herpes simplex virus
J. Gen. Virol.
53
247-255
1981
-
-
-
-
-
-
-
2
-
-
1
1
-
1
-
-
1
-
-
-
1
-
2
-
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
1
-
-
-
1
-
-
1
-
2
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
640570
Grivell
Thymidine phosphotransferase a ...
Asplenium nidus, Epidendrum hybrid, Helianthus tuberosus, Hordeum vulgare, Medicago sativa
Biochem. J.
155
571-581
1976
-
-
-
-
-
2
7
4
-
-
-
5
-
8
-
-
1
-
-
6
12
1
16
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
7
-
4
-
-
-
5
-
-
-
1
-
6
12
1
16
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-