Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | weak inhibition | Sus scrofa | |
ATP | weak inhibition | Sus scrofa | |
CMP | weak inhibition | Sus scrofa | |
dATP | 0.1 mM, 62% inhibition | Sus scrofa | |
dCMP | weak inhibition | Sus scrofa | |
dCTP | 0.1 mM, 48% inhibition | Sus scrofa | |
deoxyadenosine | weak inhibition | Sus scrofa | |
dGMP | 0.1 mM, 44% inhibition | Sus scrofa | |
dGTP | 0.1 mM, complete inhibition | Sus scrofa | |
dTMP | weak inhibition | Sus scrofa | |
dTTP | 0.1 mM, 30% inhibition | Sus scrofa | |
GMP | weak inhibition | Sus scrofa | |
GTP | 0.1 mM, 33% inhibition | Sus scrofa | |
additional information | not inhibited by dAMP | Sus scrofa | |
UMP | 0.1 mM, 24% inhibition | Sus scrofa | |
UTP | 0.1 mM, 30% inhibition | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00032 | - |
deoxyguanosine | pH 8.0, 37°C | Sus scrofa | |
3.3 | - |
ATP | pH 8.0, 37°C | Sus scrofa | |
3.3 | - |
ATP | ATP in form of MgATP2- | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation | Sus scrofa | |
Ca2+ | 67% of activity with Mg2+ | Sus scrofa | |
Fe2+ | slight activation | Sus scrofa | |
Mg2+ | required for activity | Sus scrofa | |
Mn2+ | 92% of activity with Mg2+ | Sus scrofa | |
additional information | divalent cations required, not activated by Zn2+ | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58500 | - |
gel filtration | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
piglet | - |
Purification (Comment) | Organism |
---|---|
pH 5.5, ammonium sulfate, DEAE-Sephadex | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skin | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000338 | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + deoxyguanosine | highly specific for phospho group acceptor | Sus scrofa | ADP + dGMP | - |
? | |
ATP + deoxyguanosine | ATP twice as effective as CTP or GTP and 4 times as effective as UTP | Sus scrofa | ADP + dGMP | - |
? | |
CTP + deoxyguanosine | 50% of activity with ATP | Sus scrofa | CDP + dGMP | - |
? | |
GTP + deoxyguanosine | 50% of activity with ATP | Sus scrofa | GDP + dGMP | - |
? | |
UTP + deoxyguanosine | 25% of activity with ATP | Sus scrofa | UDP + dGMP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Sus scrofa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 53 | 17% loss of activity at 53°C, 50% loss of activity at 30°C and 53°C | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | approx. 55% of maximal activity at pH 5.5 and pH 9, plateau with approx. 85% of maximal activity at pH 6.0-7.5 | Sus scrofa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
dGTP | pH 8.0, 37°C | Sus scrofa | |
0.076 | - |
dGMP | pH 8.0, 37°C | Sus scrofa | |
0.34 | - |
deoxyadenosine | pH 8.0, 37°C | Sus scrofa |