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Literature summary for 2.7.1.11 extracted from
- The effect of introducing small cavities on the allosteric inhibition of phosphofructokinase from Bacillus stearothermophilus (2016), Arch. Biochem. Biophys., 607, 1-6 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| I150V | Km-value for D-fructose 6-phosphate is 1.1fold lower than the wild-type value | Geobacillus stearothermophilus |
| I153V | mutation has a substantial positive impact on the magnitude of inhibition by phosphoenolpyruvate | Geobacillus stearothermophilus |
| I234V | Km-value for D-fructose 6-phosphate is comparable to the wild-type value | Geobacillus stearothermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphoenolpyruvate | I153V mutation has a substantial positive impact on the magnitude of inhibition by phosphoenolpyruvate | Geobacillus stearothermophilus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 100 | - |
D-fructose 6-phosphate | pH 8.0, 25°C, mutant enzyme I153V | Geobacillus stearothermophilus | |
| 110 | - |
D-fructose 6-phosphate | pH 8.0, 25°C, mutant enzyme I150V | Geobacillus stearothermophilus | |
| 126 | - |
D-fructose 6-phosphate | pH 8.0, 25°C, wild-type enzyme | Geobacillus stearothermophilus | |
| 129 | - |
D-fructose 6-phosphate | pH 8.0, 25°C, mutant enzyme I234V | Geobacillus stearothermophilus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | Geobacillus stearothermophilus | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | P00512 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | - |
Geobacillus stearothermophilus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PFK | - |
Geobacillus stearothermophilus |
| phosphofructokinase-1 | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Geobacillus stearothermophilus |
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Release 2023.1 (January 2023)
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