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Literature summary for 2.7.1.11 extracted from
- Sodium ions activated phosphofructokinase leading to enhanced D-lactic acid production by Sporolactobacillus inulinus using sodium hydroxide as a neutralizing agent (2017), Appl. Microbiol. Biotechnol., 101, 3677-3687 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ADP | allosterically activated by both GDP and ADP | Sporolactobacillus inulinus |  |
| GDP | allosterically activated by both GDP and ADP | Sporolactobacillus inulinus | |
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | sodium ions activated phosphofructokinase leads to enhanced D-lactic acid production by Sporolactobacillus inulinus using sodium hydroxide as a neutralizing agent | Sporolactobacillus inulinus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Sporolactobacillus inulinus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | above 50 mM | Sporolactobacillus inulinus | |
| phosphoenolpyruvate | - |
Sporolactobacillus inulinus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.076 | - |
ATP | pH 8.5, 30°C | Sporolactobacillus inulinus | |
| 0.48 | - |
D-fructose 6-phosphate | pH 8.5, 30°C | Sporolactobacillus inulinus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | no enzyme activity in the absence of divalent cations. Co2+ can replace Mg2+ with 35% of the maximal activity obtained in presence of Mg2+ | Sporolactobacillus inulinus | |
| K+ | activity can be enhanced by the addition of a monovalent cation (50 mM), Na+ (relative activity 133%), K+ (117%), and NH4+ (114%) in the presence of Mg2+ | Sporolactobacillus inulinus | |
| Mg2+ | no enzyme activity can be detected in the absence of divalent cations. Mg2+ is the most efficient divalent tested to support the reaction. The enzyme is absolutely dependent on divalent cations, forming a complex with a phosphoryl donor | Sporolactobacillus inulinus | |
| Mn2+ | no enzyme activity in the absence of divalent cations. Co2+ can replace Mg2+ with 79% of the maximal activity obtained in presence of Mg2+ | Sporolactobacillus inulinus | |
| additional information | no effect: Ni2+, Zn2+, Cu2+, Ca2+, and Fe2+ | Sporolactobacillus inulinus | |
| Na+ | activity can be enhanced by the addition of a monovalent cation (50 mM), Na+ (relative activity 133%), K+ (117%), and NH4+ (114%) in the presence of Mg2+. Activity is significantly increased with the increased concentration of NaCl up to 50 mM | Sporolactobacillus inulinus | |
| NH4+ | activity can be enhanced by the addition of a monovalent cation (50 mM), Na+ (relative activity 133%), K+ (117%), and NH4+ (114%) in the presence of Mg2+ | Sporolactobacillus inulinus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
SDS-PAGE | Sporolactobacillus inulinus |
| 34380 | - |
calculated from sequence | Sporolactobacillus inulinus |
| 135000 | - |
gel filtration | Sporolactobacillus inulinus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | Sporolactobacillus inulinus | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
| ATP + D-fructose 6-phosphate | Sporolactobacillus inulinus Y2-8 | - |
ADP + D-fructose 1,6-bisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sporolactobacillus inulinus | - |
- |
- |
| Sporolactobacillus inulinus Y2-8 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sporolactobacillus inulinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | - |
Sporolactobacillus inulinus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
| ATP + D-fructose 6-phosphate | the enzyme shows strictly high specificity for its phosphoryl donor. ADP, AMP, GDP, and PPi could not substitute for ATP | Sporolactobacillus inulinus | ADP + D-fructose 1,6-bisphosphate | - |
? | |
| ATP + D-fructose 6-phosphate | - |
Sporolactobacillus inulinus Y2-8 | ADP + D-fructose 1,6-bisphosphate | - |
? | |
| ATP + D-fructose 6-phosphate | the enzyme shows strictly high specificity for its phosphoryl donor. ADP, AMP, GDP, and PPi could not substitute for ATP | Sporolactobacillus inulinus Y2-8 | ADP + D-fructose 1,6-bisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 34000, SDS-PAGE | Sporolactobacillus inulinus |
| homotetramer | 4 * 34380, calculated from sequence | Sporolactobacillus inulinus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP-dependent PFK | - |
Sporolactobacillus inulinus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Sporolactobacillus inulinus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | broad temperature optimum with at least 90% of the maximal activity retained between 20 and 40 °C | Sporolactobacillus inulinus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Sporolactobacillus inulinus |
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