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Literature summary for 2.7.1.11 extracted from

  • Le, S.B.; Heggeset, T.M.B.; Haugen, T.; Naerdal, I.; Brautaset, T.
    6-Phosphofructokinase and ribulose-5-phosphate 3-epimerase in methylotrophic Bacillus methanolicus ribulose monophosphate cycle (2017), Appl. Microbiol. Biotechnol., 101, 4185-4200 .
    View publication on PubMed
Search for more literature for 2.7.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus methanolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
 ATP strain PB1, Pfk2, pH 9.0, 30°C Bacillus methanolicus
0.39
-
 ATP strain MGA3, Pfk2, pH 9.0, 30°C Bacillus methanolicus
0.61
-
 D-fructose 6-phosphate strain MGA3, Pfk2, pH 9.0, 30°C Bacillus methanolicus
0.79
-
 D-fructose 6-phosphate strain PB1, Pfk2, pH 9.0, 30°C Bacillus methanolicus
0.82
-
 ATP strain MGA3, Pfk1, pH 9.0, 30°C Bacillus methanolicus
0.94
-
 D-fructose 6-phosphate strain MGA3, Pfk1, pH 9.0, 30°C Bacillus methanolicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates Bacillus methanolicus
Mn2+ stimulates Bacillus methanolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose 6-phosphate Bacillus methanolicus the enzyme is involved in the ribulose monophosphate cycle ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate Bacillus methanolicus PB1 the enzyme is involved in the ribulose monophosphate cycle ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate Bacillus methanolicus MGA3 the enzyme is involved in the ribulose monophosphate cycle ADP + D-fructose 1,6-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus methanolicus
-
-
-
Bacillus methanolicus I3DYB8 strain PB1, pfk2
-
Bacillus methanolicus MGA3
-
-
-
Bacillus methanolicus PB1
-
-
-
Bacillus methanolicus PB1 I3DYB8 strain PB1, pfk2
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus methanolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Bacillus methanolicus ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate the enzyme is involved in the ribulose monophosphate cycle Bacillus methanolicus ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate
-
 Bacillus methanolicus PB1 ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate the enzyme is involved in the ribulose monophosphate cycle Bacillus methanolicus PB1 ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate
-
 Bacillus methanolicus MGA3 ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate the enzyme is involved in the ribulose monophosphate cycle Bacillus methanolicus MGA3 ADP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
octamer active both as octamer and as tetramer Bacillus methanolicus
tetramer active both as octamer and as tetramer Bacillus methanolicus

Synonyms

Synonyms Comment Organism
PFK
-
 Bacillus methanolicus
PFK1 wild-type strain MGA3, encoded on plasmid pBM19 Bacillus methanolicus
PFK1 wild-type strain PB1, encoded on plasmid pBM20 Bacillus methanolicus
PFK2 wild-type strain MGA3, chromosomal enzyme Bacillus methanolicus
PFK2 wild-type strain PB1, chromosomal enzyme Bacillus methanolicus

Expression

Organism Comment Expression
Bacillus methanolicus transcription of the plasmid-encoded gene is 10fold to 15fold upregulated in cells growing on methanol compared to mannitol, while the chromosomal gene is transcribed at similar levels under both conditions up

General Information

General Information Comment Organism
physiological function the enzyme is involved in the ribulose monophosphate cycle Bacillus methanolicus