Application | Comment | Organism |
---|---|---|
medicine | somatic PFK1 mutations identified in human cancers have distinct effects on allosteric regulation of enzymic activity and lactate production | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
structures of the mammalian 6-phosphofructokinase PFK1 tetramer, for the human platelet isoform, in complex with MgATPMg2- and ADP at 3.1 and 3.4 A resolution, respectively. The structures reveal substantial conformational changes in the enzyme upon nucleotide hydrolysis as well as a unique tetramer interface. Mutations of residues in this interface can affect tetramer formation, enzyme catalysis and regulation. Somatic PFK1 mutations identified in human cancers have distinct effects on allosteric regulation of enzymic activity and lactate production | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D564N | somatic mutation identified in human cancers. Mutant has decreased maximum velocity and affinity for fructose 6-phosphate | Homo sapiens |
E657A | mutant has reduced affinity of about 4.5 mM for frucotse 6-phosphate, compared with about 0.8 mM in wild type, and an 2fold decrease in maximum activity | Homo sapiens |
N426S | somatic mutation identified in human cancers. Mutation partially relieves ATP inhibition | Homo sapiens |
R48C | somatic mutation identified in human cancers. Residue Arg48 interacts with a bound phosphate ion in the structure, and the mutant shows reduced citrate inhibition | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q01813 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood platelet | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
PFK1 | - |
Homo sapiens |