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Literature summary for 2.7.1.11 extracted from

  • Brueser, A.; Kirchberger, J.; Kloos, M.; Straeter, N.; Schoeneberg, T.
    Functional linkage of adenine nucleotide binding sites in mammalian muscle 6-phosphofructokinase (2012), J. Biol. Chem., 287, 17546-17553.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.11

Activating Compound

Activating Compound Comment Organism Structure
ADP
-
 Homo sapiens
AMP
-
 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain HD114-8D Homo sapiens

Protein Variants

Protein Variants Comment Organism
H242A the mutant enzyme shows reduced Km value for ATP and 38.4% residual activity in the presence of 4.5 mM ADP Homo sapiens
K386A the mutant enzyme shows increased Km value for ATP and 33.1% residual activity in the presence of 4.5 mM ADP Homo sapiens
K678A the mutant enzyme shows reduced Km value for ATP. Ki values of ADP and ATP remain unchanged Homo sapiens
N341A the mutant enzyme shows reduced Km value for ATP. Ki values of ADP and ATP are significantly reduced in this mutant Homo sapiens
N341A/R246A/K386A the mutant enzyme shows reduced Km value for ATP and 21.9% residual activity in the presence of 4.5 mM ADP Homo sapiens
R246A the mutant enzyme shows slightly increased Km value for ATP and 41.7% residual activity in the presence of 4.5 mM ADP Homo sapiens
R246A/K386A the mutant enzyme shows increased Km value for ATP and 33.2% residual activity in the presence of 4.5 mM ADP Homo sapiens
S377A the mutant enzyme shows reduced Km value for ATP. Ki values of ADP and ATP remain unchanged Homo sapiens
S377A/K678A the mutant enzyme shows reduced Km value for ATP. Ki values of ADP and ATP are significantly reduced in this mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ADP complete inhibition at 4.5 mM ADP, 52.4% residual activity at 2.3 mM ADP Homo sapiens
ATP millimolar inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0151
-
 ATP mutant enzyme N341A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.0232
-
 ATP mutant enzyme N341A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.0275
-
 ATP mutant enzyme S377A/K678A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.0326
-
 ATP mutant enzyme K678A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.0338
-
 ATP mutant enzyme H242A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.0356
-
 ATP mutant enzyme K386A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.0356
-
 ATP mutant enzyme N341A/R246A/K386A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.037
-
 ATP mutant enzyme S377A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.0382
-
 ATP mutant enzyme H242A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.0384
-
 ATP mutant enzyme K678A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.0415
-
 ATP mutant enzyme S377A/K678A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.0425
-
 ATP mutant enzyme R246A/K386A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.0425
-
 ATP wild type enzyme, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.043
-
 ATP mutant enzyme S377A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.0432
-
 ATP mutant enzyme R246A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.0477
-
 ATP mutant enzyme R246A/K386A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.0498
-
 ATP wild type enzyme, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.0508
-
 ATP mutant enzyme R246A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.0524
-
 ATP mutant enzyme K386A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.0528
-
 ATP mutant enzyme N341A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.063
-
 ATP mutant enzyme N341A/R246A/K386A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.114
-
 ATP mutant enzyme H242A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.141
-
 ATP mutant enzyme S377A/K678A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.166
-
 ATP mutant enzyme R246A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.168
-
 ATP mutant enzyme K386A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.168
-
 ATP mutant enzyme S377A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.169
-
 ATP wild type enzyme, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.22
-
 ATP mutant enzyme N341A/R246A/K386A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.234
-
 ATP mutant enzyme R246A/K386A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
0.25
-
 ATP mutant enzyme K678A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Cibacron Blue F3GA-Sephadex G75 gel filtration, Resource Q column chromatography, and BioSep SEC-S4000 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Homo sapiens ADP + D-fructose 1,6-bisphosphate
-
 ?

Synonyms

Synonyms Comment Organism
ATP-dependent 6-phosphofructokinase
-
 Homo sapiens
ATP: D-fructose-6-phosphate-1-phosphotransferase
-
 Homo sapiens
PFK
-
 Homo sapiens
phosphofructokinase-1
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3
-
 ADP mutant enzyme N341A, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
1.4
-
 ATP mutant enzyme N341A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
1.6
-
 ADP mutant enzyme S377A/K678A, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
1.6
-
 ATP mutant enzyme S377A/K678A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
1.9
-
 ATP mutant enzyme K678A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
2
-
 ADP mutant enzyme K678A, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
2
-
 ATP wild type enzyme, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
2.1
-
 ATP mutant enzyme N341A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
2.1
-
 ADP wild type enzyme, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
2.2
-
 ATP mutant enzyme S377A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
2.3
-
 ADP mutant enzyme N341A/R246A/K386A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
2.4
-
 ADP mutant enzyme S377A, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
2.5
-
 ADP mutant enzyme R246A/K386A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
3.1
-
 ATP mutant enzyme S377A/K678A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
3.2
-
 ADP mutant enzyme H242A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
3.2
-
 ADP mutant enzyme K386A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
3.4
-
 ADP mutant enzyme R246A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
4.3
-
 ATP mutant enzyme K678A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
5.2
-
 ATP mutant enzyme N341A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
5.2
-
 ATP mutant enzyme S377A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
5.3
-
 ATP wild type enzyme, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
5.6
-
 ATP mutant enzyme K386A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
5.8
-
 ATP mutant enzyme S377A/K678A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
5.9
-
 ATP mutant enzyme N341A/R246A/K386A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
6.8
-
 ATP mutant enzyme H242A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
7.6
-
 ATP mutant enzyme N341A/R246A/K386A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
8.1
-
 ATP mutant enzyme K678A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
8.5
-
 ATP wild type enzyme, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
8.6
-
 ATP mutant enzyme R246A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
8.6
-
 ATP mutant enzyme S377A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
8.8
-
 ATP mutant enzyme N341A/R246A/K386A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
9.5
-
 ATP mutant enzyme K386A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
10
-
 ATP Ki above 10 mM, mutant enzyme H242A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
10
-
 ATP Ki above 10 mM, mutant enzyme H242A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
10
-
 ATP Ki above 10 mM, mutant enzyme K386A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
10
-
 ATP Ki above 10 mM, mutant enzyme R246A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
10
-
 ATP Ki above 10 mM, mutant enzyme R246A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
10
-
 ATP Ki above 10 mM, mutant enzyme R246A/K386A, in the presence of 0.82 mM ADP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
10
-
 ATP Ki above 10 mM, mutant enzyme R246A/K386A, in the presence of 1 mM AMP, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens
10
-
 ATP Ki above 10 mM, mutant enzyme R246A/K386A, without effectors, in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2, pH 7.0, at 25°C Homo sapiens