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Literature summary for 2.7.1.11 extracted from
- Purification, molecular and kinetic characterization of phosphofructokinase-1 from the yeast Schizosaccharomyces pombe: evidence for an unusual subunit composition (2000), Yeast, 16, 1273-1285.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| AMP | activation follows a saturation function, Km: 0.56 mM | Schizosaccharomyces pombe |  |
| D-fructose 2,6-bisphosphate | cooperative activation, Km: 0.00023 mM | Schizosaccharomyces pombe | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | lower inhibition at pH 6.6 compared to pH 7.2 | Schizosaccharomyces pombe | |
| phosphate | 25 mM, 50% and 57% inhibition of PFK-1 at pH 6.6 and pH 7.2, respectively | Schizosaccharomyces pombe | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.027 | - |
ATP | pH 7.2, 25°C | Schizosaccharomyces pombe | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 103306 | - |
8 * 103306, MALDI-TOF mass spectrometry | Schizosaccharomyces pombe |
| 790000 | - |
sedimentation equilibrium | Schizosaccharomyces pombe |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizosaccharomyces pombe | - |
PFK-1 | - |
| Schizosaccharomyces pombe CBS1057 | - |
PFK-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| polyethylene glycol 6000, F3G-A-Sephadex, Resource Q | Schizosaccharomyces pombe |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 136 | - |
PFK-1 | Schizosaccharomyces pombe |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | sigmoidal kinetics with fructose 6-phosphate | Schizosaccharomyces pombe | ADP + D-fructose 1,6-bisphosphate | - |
? | |
| ATP + D-fructose 6-phosphate | sigmoidal kinetics with fructose 6-phosphate | Schizosaccharomyces pombe CBS1057 | ADP + D-fructose 1,6-bisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 103306, MALDI-TOF mass spectrometry | Schizosaccharomyces pombe |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
80% of maximal activity at pH 6.6 | Schizosaccharomyces pombe |
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