Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | activation follows a saturation function, Km: 0.56 mM | Schizosaccharomyces pombe | |
D-fructose 2,6-bisphosphate | cooperative activation, Km: 0.00023 mM | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | lower inhibition at pH 6.6 compared to pH 7.2 | Schizosaccharomyces pombe | |
phosphate | 25 mM, 50% and 57% inhibition of PFK-1 at pH 6.6 and pH 7.2, respectively | Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
ATP | pH 7.2, 25°C | Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
103306 | - |
8 * 103306, MALDI-TOF mass spectrometry | Schizosaccharomyces pombe |
790000 | - |
sedimentation equilibrium | Schizosaccharomyces pombe |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
PFK-1 | - |
Schizosaccharomyces pombe CBS1057 | - |
PFK-1 | - |
Purification (Comment) | Organism |
---|---|
polyethylene glycol 6000, F3G-A-Sephadex, Resource Q | Schizosaccharomyces pombe |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
136 | - |
PFK-1 | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | sigmoidal kinetics with fructose 6-phosphate | Schizosaccharomyces pombe | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | sigmoidal kinetics with fructose 6-phosphate | Schizosaccharomyces pombe CBS1057 | ADP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 103306, MALDI-TOF mass spectrometry | Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
80% of maximal activity at pH 6.6 | Schizosaccharomyces pombe |