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Literature summary for 2.7.1.11 extracted from

  • Hansen, T.; Musfeldt, M.; Schonheit, P.
    ATP-dependent 6-phosphofructokinase from the hyperthermophilic bacterium Thermotoga maritima: characterization of an extremely thermophilic, allosterically regulated enzyme (2002), Arch. Microbiol., 177, 401-409.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP
-
Thermotoga maritima

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
phosphoenolpyruvate 0.1 mM and 10 mM, allosteric inhibition, almost completely reversed by 2.5 mM ADP Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
ATP pH 7.0, 50°C and 75°C Thermotoga maritima
1.4
-
ADP pH 7.0, 75°C Thermotoga maritima
7.6
-
D-fructose 1,6-bisphosphate pH 7.0, 75°C Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 30% of activity with Mg2+ Thermotoga maritima
Mg2+ required for activity Thermotoga maritima
Mn2+
-
Thermotoga maritima
Zn2+ 70% of activity with Mg2+ Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
alpha4, 4 * 34000, SDS-PAGE Thermotoga maritima
140000
-
gel filtration Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-fructose 6-phosphate Thermotoga maritima
-
ADP + D-fructose 1,6-bisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose, ATP-agarose, Uno S1 Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
62
-
at 50°C Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
Thermotoga maritima ADP + D-fructose 1,6-bisphosphate
-
?
ATP + D-fructose 6-phosphate specific for fructose 6-phosphate, very low activity with UTP and CTP Thermotoga maritima ADP + D-fructose 1,6-bisphosphate
-
r
ATP + D-fructose 6-phosphate reversible reaction in vitro Thermotoga maritima ADP + D-fructose 1,6-bisphosphate
-
r
GTP + D-fructose 6-phosphate 64% of activity of ATP Thermotoga maritima GDP + D-fructose 1,6-bisphosphate
-
?
ITP + D-fructose 6-phosphate 38% of activity with ATP Thermotoga maritima IDP + D-fructose 1,6-bisphosphate
-
?

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 34000, SDS-PAGE Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
93
-
-
Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 100 very low activity at 20°C, exponential increase in activity between 40°C and 93°C, approx. 50% of maximal activity at 80°C, activation energy: 54 kJ/mol Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
less than 20% loss of activity after 2 h Thermotoga maritima
80
-
less than 20% loss of activity after 2 h Thermotoga maritima
95
-
complete loss of activity after 1 h Thermotoga maritima
100
-
complete loss of activity after 20 min, 40% loss of activity after 2 h in the presence of 1 M ammonium sulfate Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
forward direction, at 75°C, 50% of maximal activity at pH 5.7, 80% at pH 7.7 Thermotoga maritima