Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Dimethylsulfoxide | 20%, 9fold increase in activity, probably due to crowding effect | Oncorhynchus mykiss | |
glycerol | 40%, 4fold increase in activity, probably due to crowding effect | Oncorhynchus mykiss | |
Polyethylene glycol | 20%, 8fold increase in activity, probably due to crowding effect | Oncorhynchus mykiss |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 0.02 mM and 0.94 mM, 50% inhibition at 0.0001 mg/ml and at 0.0006 mg/ml PFK respectively, 3.23 mM, 50% inhibition at 0.0006 mg/ml PFK in the presence of 10% polyethylene glycol | Oncorhynchus mykiss |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oncorhynchus mykiss | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
white muscle | - |
Oncorhynchus mykiss | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity increases with increasing amounts of PFK, approx. 50% of maximal spec. activity at approx. 0.001 mg/ml, maximal activity at approx. 0.0035 mg/ml | Oncorhynchus mykiss |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
optimum is shifted to approx. pH 7.5 in the presence of polyethylene glycol | Oncorhynchus mykiss |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | less than 5% of maximal activity at pH 7.0, approx. 50% of maximal activity at pH 7.5, pH curve is shifted to the left in the presence of 10% polyethylene glycol | Oncorhynchus mykiss |