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Literature summary for 2.7.1.11 extracted from

  • Su, J.Y.; Storey, K.B.
    Fish muscle phosphofructokinase: influences of protein concentration on enzyme kinetic behavior (1995), Int. J. Biochem. Cell Biol., 27, 1277-1283.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
Dimethylsulfoxide 20%, 9fold increase in activity, probably due to crowding effect Oncorhynchus mykiss
glycerol 40%, 4fold increase in activity, probably due to crowding effect Oncorhynchus mykiss
Polyethylene glycol 20%, 8fold increase in activity, probably due to crowding effect Oncorhynchus mykiss

Inhibitors

Inhibitors Comment Organism Structure
ATP 0.02 mM and 0.94 mM, 50% inhibition at 0.0001 mg/ml and at 0.0006 mg/ml PFK respectively, 3.23 mM, 50% inhibition at 0.0006 mg/ml PFK in the presence of 10% polyethylene glycol Oncorhynchus mykiss

Organism

Organism UniProt Comment Textmining
Oncorhynchus mykiss
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
white muscle
-
Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activity increases with increasing amounts of PFK, approx. 50% of maximal spec. activity at approx. 0.001 mg/ml, maximal activity at approx. 0.0035 mg/ml Oncorhynchus mykiss

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
optimum is shifted to approx. pH 7.5 in the presence of polyethylene glycol Oncorhynchus mykiss

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5 less than 5% of maximal activity at pH 7.0, approx. 50% of maximal activity at pH 7.5, pH curve is shifted to the left in the presence of 10% polyethylene glycol Oncorhynchus mykiss