Literature summary for 2.7.1.11 extracted from

Zhu, X.; Byrnes, M.; Nelson, J.W.; Chang, S.H.
Role of glycine 212 in the allosteric behavior of phosphofructokinase from Bacillus stearothermophilus (1995), Biochemistry, 34, 2560-2565.

Search for more literature for 2.7.1.11

Activating Compound

Activating Compound	Comment	Organism	Structure
GDP	activation at low concetrations, inhibition above 1 mM	Geobacillus stearothermophilus

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and G212V mutant PFK in Escherichia coli	Geobacillus stearothermophilus

Protein Variants

Protein Variants	Comment	Organism
G212V	-	Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.023	-	D-fructose 6-phosphate	pH 8.2, 30°C, native PFK	Geobacillus stearothermophilus
0.026	-	D-fructose 6-phosphate	pH 8.2, 30°C, G212V mutant PFK	Geobacillus stearothermophilus
0.12	-	ATP	pH 8.2, 30°C, native PFK	Geobacillus stearothermophilus
0.13	-	ATP	pH 8.2, 30°C, G212V mutant PFK	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-fructose 6-phosphate	-	Geobacillus stearothermophilus	ADP + D-fructose 1,6-bisphosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
126	-	fructose 6-phosphate	pH 8.2, 30°C, G212V mutant PFK	Geobacillus stearothermophilus
149	-	fructose 6-phosphate	pH 8.2, 30°C, native PFK	Geobacillus stearothermophilus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)