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Literature summary for 2.7.1.11 extracted from

  • Baumann, L.; Baumann, P.
    Catabolism of D-fructose and D-ribose by Pseudomonas doudoroffii. II. Properties of 1-phosphofructokinase and 6-phosphofructokinase (1975), Arch. Microbiol., 105, 241-248.
    View publication on PubMed
Search for more literature for 2.7.1.11

Activating Compound

Activating Compound Comment Organism Structure
ADP activation Oceanimonas doudoroffii
ADP positive effector Oceanimonas doudoroffii
NH4+ required for activity Oceanimonas doudoroffii

Inhibitors

Inhibitors Comment Organism Structure
ATP above 0.25 mM in the absence of MgCl2; free form Oceanimonas doudoroffii
citrate cAMP, ADP or fructose 1,6-bisphosphate restore activity Oceanimonas doudoroffii
additional information not inhibited by AMP; not inhibited by cAMP; not inhibited by phosphate; not inhibited by pyruvate Oceanimonas doudoroffii
phosphoenolpyruvate
-
 Oceanimonas doudoroffii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 ATP pH 8.0, 28°C Oceanimonas doudoroffii
0.15
-
 ATP
-
 Oceanimonas doudoroffii

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required for activity, inhibition at high concentrations is reversed by increased concentrations of ATP Oceanimonas doudoroffii
Mg2+ required for activity Oceanimonas doudoroffii
Mg2+ 1-10 mM are required for maximal activity Oceanimonas doudoroffii
Mn2+ as effective as Mg2+ Oceanimonas doudoroffii
Mn2+ activation Oceanimonas doudoroffii

Organism

Organism UniProt Comment Textmining
Oceanimonas doudoroffii
-
-
-
Oceanimonas doudoroffii M1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Oceanimonas doudoroffii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5 3.1
-
 Oceanimonas doudoroffii

Storage Stability

Storage Stability Organism
-15°C, 3 days, complete loss of activity Oceanimonas doudoroffii
4°C, 1 week, 47% loss of activity Oceanimonas doudoroffii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Oceanimonas doudoroffii ADP + D-fructose 1,6-bisphosphate
-
 ?
ATP + D-fructose 6-phosphate
-
 Oceanimonas doudoroffii M1 ADP + D-fructose 1,6-bisphosphate
-
 ?
CTP + D-fructose 6-phosphate phosphorylation at 28% the rate of ATP Oceanimonas doudoroffii CDP + D-fructose 1,6-bisphosphate
-
 ?
CTP + D-fructose 6-phosphate phosphorylation at 28% the rate of ATP Oceanimonas doudoroffii M1 CDP + D-fructose 1,6-bisphosphate
-
 ?
GTP + D-fructose 6-phosphate phosphorylation at 35% the rate of ATP Oceanimonas doudoroffii GDP + D-fructose 1,6-bisphosphate
-
 ?
GTP + D-fructose 6-phosphate phosphorylation at 35% the rate of ATP Oceanimonas doudoroffii M1 GDP + D-fructose 1,6-bisphosphate
-
 ?
ITP + D-fructose 6-phosphate phosphorylation at 71% the rate of ATP Oceanimonas doudoroffii IDP + D-fructose 1,6-bisphosphate
-
 ?
ITP + D-fructose 6-phosphate phosphorylation at 71% the rate of ATP Oceanimonas doudoroffii M1 IDP + D-fructose 1,6-bisphosphate
-
 ?
UTP + D-fructose 6-phosphate phosphorylation at 16% the rate of ATP Oceanimonas doudoroffii UDP + D-fructose 1,6-bisphosphate
-
 ?
UTP + D-fructose 6-phosphate phosphorylation at 16% the rate of ATP Oceanimonas doudoroffii M1 UDP + D-fructose 1,6-bisphosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Oceanimonas doudoroffii

pH Range

pH Minimum pH Maximum Comment Organism
7 8 approx. 60% of maximal activity at pH 7, approx 95% of maximal activity at pH 8 Oceanimonas doudoroffii