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Literature summary for 2.7.1.11 extracted from

  • Layzer, R.B.
    Phosphofructokinase from human erythrocytes (1975), Methods Enzymol., 42C, 110-115.
No PubMed abstract available
Search for more literature for 2.7.1.11

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
 Homo sapiens
citrate weak inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic study Homo sapiens
0.12
-
 ATP pH 8.0, 25°C, erythrocyte PFK Homo sapiens
0.45
-
 D-fructose 6-phosphate pH 8.0, 25°C, erythrocyte PFK Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
 x * 76000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
batch DEAE-cellulose, ammonium sulfate, heat, DEAE-cellulose, Blue Dextran Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
90
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
5°C, 50 mM potassium phosphate buffer, pH 8, 1 mM EDTA, 1 mM AMP, 1 mM dithiothreitol, 2 mM fructose 6-phosphate, 30% loss of activity per month Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Homo sapiens ADP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 76000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens