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Literature summary for 2.7.1.11 extracted from

  • Brooks, S.P.J.; Storey, K.B.
    Phosphofructokinase from a vertebrate facultative anaerobe: effects of temperature and anoxia on the kinetic parameters of the purified enzyme from turtle white muscle (1990), Biochim. Biophys. Acta, 1037, 161-164.
    View publication on PubMed
Search for more literature for 2.7.1.11

Inhibitors

Inhibitors Comment Organism Structure
ATP 0.004 mM and 0.003 mM, 50% inhibition at 20°C and 6°C, respectively, normoxic turtles Trachemys scripta
citrate 0.23 and 0.17 mM mM, 50% inhibition at 20° and 6°C, respectively, normoxic turtles, 0.3 mM, 50% inhibition at 20°C and 6°C, anoxic turtles Trachemys scripta
additional information effect of low temperature or anoxia on inhibition kinetics Trachemys scripta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information effects of low temperature and anoxia on kinetic constants Trachemys scripta
0.023
-
 ATP pH 7.0, 6°C, normoxic animals Trachemys scripta
0.03
-
 ATP pH 7.0, 6°C, anoxic animals Trachemys scripta
0.034
-
 ATP pH 7.0, 20°C, normoxic and anoxic animals Trachemys scripta
0.043
-
 D-fructose 6-phosphate pH 7.0, 6°C, normoxic animals Trachemys scripta
0.095
-
 D-fructose 6-phosphate pH 7.0, 6°C, anoxic animals Trachemys scripta
0.106
-
 D-fructose 6-phosphate pH 7.0, 20°C, anoxic animals Trachemys scripta
0.11
-
 D-fructose 6-phosphate pH 7.0, 20°C, normoxic animals Trachemys scripta

Organism

Organism UniProt Comment Textmining
Trachemys scripta
-
 freshwater turtle
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trachemys scripta

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Trachemys scripta
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
115
-
-
 Trachemys scripta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Trachemys scripta ADP + D-fructose 1,6-bisphosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6
-
 assay at Trachemys scripta
20
-
 assay at Trachemys scripta