Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.11 extracted from

  • Su, Y.; Storey, K.B.
    Phosphofructokinase from white muscle of the rainbow trout, Oncorhynchus mykiss: purification and properties (1992), Biochim. Biophys. Acta, 1160, 301-308.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
AMP activation Oncorhynchus mykiss
D-fructose 2,6-bisphosphate activation Oncorhynchus mykiss

General Stability

General Stability Organism
labile at all stages of purification, particularly at high dilutions or high salt concentrations, glycerol, fructose 6-phosphate and high enzyme concentrations stabilize Oncorhynchus mykiss

Inhibitors

Inhibitors Comment Organism Structure
ATP 0.99 mM, 50% inhibition Oncorhynchus mykiss
citrate 0.12 mM, 50% inhibition Oncorhynchus mykiss
phosphoenolpyruvate 0.59 mM, 50% inhibition Oncorhynchus mykiss

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
ATP pH 7.2, 22°C Oncorhynchus mykiss

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76500
-
6 * 76500, SDS-PAGE Oncorhynchus mykiss
478000
-
gel filtration Oncorhynchus mykiss

Organism

Organism UniProt Comment Textmining
Oncorhynchus mykiss
-
rainbow trout
-

Purification (Commentary)

Purification (Comment) Organism
Cellulose-P, hydroxyapatite, ATP-agarose Oncorhynchus mykiss

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Oncorhynchus mykiss
-
skeletal muscle white skeletal muscle Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
76.5
-
-
Oncorhynchus mykiss

Storage Stability

Storage Stability Organism
-20°C, 0.06 mg protein/ml, in 20 mM phosphate buffer, pH 7.5, 40% v/v glycerol, 1 month Oncorhynchus mykiss

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
Oncorhynchus mykiss ADP + D-fructose 1,6-bisphosphate
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 76500, SDS-PAGE Oncorhynchus mykiss

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Oncorhynchus mykiss

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 AMP, fructose 2,6-bisphosphate, and phosphate lower the pH optimum to approx. pH 7.6, 7.8 and 7.6, respectively Oncorhynchus mykiss

pH Range

pH Minimum pH Maximum Comment Organism
7.4 8.5 approx. half-maximal activity at pH 7.4, maximal activity at pH 8.5, activators shift the optimum to lower pH-values Oncorhynchus mykiss

pI Value

Organism Comment pI Value Maximum pI Value
Oncorhynchus mykiss isoelectric focusing
-
7.1