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Literature summary for 2.7.1.11 extracted from

  • Stellwagen, E.; Wilgus, H.
    Phosphofructokinase from rabbit skeletal muscle (1975), Methods Enzymol., 42C, 78-85.
    View publication on PubMed
Search for more literature for 2.7.1.11

Activating Compound

Activating Compound Comment Organism Structure
AMP activation Saccharomyces cerevisiae
AMP positive effector Saccharomyces cerevisiae
additional information K-type allosteric enzyme Saccharomyces cerevisiae
additional information allosteric enzyme Saccharomyces cerevisiae
NH4+ activation at low concentrations Saccharomyces cerevisiae

General Stability

General Stability Organism
fructose 6-phosphate, ATP or ammonium sulfate stabilizes during dialysis and storage at pH 6-9 Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
ATP NaF and limited proteolysis protect from inhibition Saccharomyces cerevisiae
citrate
-
 Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activation at low concentrations Saccharomyces cerevisiae
Mg2+ required for activity Saccharomyces cerevisiae
Mg2+ MgATP is the active substrate Saccharomyces cerevisiae
additional information K-type allosteric enzyme Saccharomyces cerevisiae
additional information not activated by Na+ and Rb+ Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate, ammonium sulfate, acetone, ammonium sulfate, Bio-Gel, DEAE-cellulose, hydroxyapatite Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
ATP + beta-D-fructofuranose 6-phosphate = ADP + beta-D-fructofuranose 1,6-bisphosphate K-type allosteric enzyme, ordered sequential mechanism at pH 6.4 Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
148
-
-
 Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-15°C, at least 4 weeks Saccharomyces cerevisiae
4°C, fructose 6-phosphate, ATP or NH4+ stabilizes at pH-values from 6 to 9 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Saccharomyces cerevisiae ADP + D-fructose 1,6-bisphosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 fructose 6-phosphate, ATP or NH4+ stabilizes at 4°C Saccharomyces cerevisiae