Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | activation | Lactococcus lactis | |
NH4+ | - |
Lactococcus lactis |
Crystallization (Comment) | Organism |
---|---|
4 mg/ml PFK in 25 mM sodium phosphate buffer pH 7.0, is dialyzed at 4°C against 25 mM phosphate buffer, pH 7.0, containing 10 mM 2-mercaptoethanol, 1 mM MgSO4, 1 mM EDTA, 2 mM ATP, 2 mM FDP and 23% ammonium sulfate, crystals appear after 8-10 weeks | Lactococcus lactis |
General Stability | Organism |
---|---|
dilution inactivates | Lactococcus lactis |
fructose 6-phosphate, ATP, ADP, citrate, KCl or ammonium sulfate does not stabilize | Lactococcus lactis |
glycerol prevents inactivation of dilute cytosolic enzyme solution | Lactococcus lactis |
glycerol stabilizes | Lactococcus lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | 5 mM, 80% inhibition | Lactococcus lactis | |
D-fructose 1,6-bisphosphate | 5 mM, 25% inhibition | Lactococcus lactis | |
Li+ | weak | Lactococcus lactis | |
Na+ | weak | Lactococcus lactis | |
phosphate | 10 mM, 50% inhibition | Lactococcus lactis | |
phosphoenolpyruvate | 5 mM; 64% inhibition | Lactococcus lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
ATP | pH 7.5 | Lactococcus lactis | |
0.18 | - |
ATP | ATP in form of MgATP2- | Lactococcus lactis | |
0.25 | - |
D-fructose 6-phosphate | pH 7.5 | Lactococcus lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 50% of activity with Mg2+- | Lactococcus lactis | |
K+ | slight activation at low concentrations | Lactococcus lactis | |
Mg2+ | required for activity | Lactococcus lactis | |
Mg2+ | maximal activity at 2-5 mM | Lactococcus lactis | |
Mn2+ | activation | Lactococcus lactis | |
Mn2+ | 75% of activity with Mg2+ | Lactococcus lactis | |
Ni2+ | activation, 12% of activity with | Lactococcus lactis | |
Zn2+ | activation | Lactococcus lactis | |
Zn2+ | 35% of activity with Mg2+ | Lactococcus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
144500 | - |
gel filtration | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
- |
- |
Lactococcus lactis C10 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lactococcus lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
161 | - |
- |
Lactococcus lactis |
Storage Stability | Organism |
---|---|
-12°C, Tris-glycerol buffer, pH 7.5, 50% v/v glycerol, up to 6 months, 10-20% loss of activity | Lactococcus lactis |
4°C, less stable at pH 6 and 8 than at neutral pH-values | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Lactococcus lactis | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Lactococcus lactis C10 | ADP + D-fructose 1,6-bisphosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.8 | - |
Lactococcus lactis |