Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | 1.8 mM, half-maximal activation at fructose 6-phosphate concentrations below 0.5 mM | Trypanosoma brucei | |
AMP | 0.64 mM, half-maximal activation at fructose 6-phosphate concentrations below 0.5 mM | Trypanosoma brucei | |
cAMP | 0.4 mM, half-maximal activation at fructose 6-phosphate concentrations below 0.5 mM | Trypanosoma brucei |
General Stability | Organism |
---|---|
AMP, ADP, fructose 6-phosphate or antipain stabilizes during purification | Trypanosoma brucei |
concentration by ultrafiltration on Amicon PM-10 membrane results in appreciable loss of activity, concentration in collodium bags allows higher protein concentration to be obtained without loss of activity | Trypanosoma brucei |
dilution leads to enzyme dissociation | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | kinetics | Trypanosoma brucei | |
AMP | kinetics | Trypanosoma brucei | |
citrate | cAMP, ADP or fructose 1,6-bisphosphate restore activity | Trypanosoma brucei | |
additional information | not inhibited by fructose 1,6-bisphosphate; not inhibited by fructose 2,6-bisphosphate; not inhibited by phosphate | Trypanosoma brucei | |
phosphoenolpyruvate | inhibition kinetic | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Trypanosoma brucei | |
0.013 | - |
ATP | pH 6.7, 37°C, at 0.25 mM fructose 6-phosphate | Trypanosoma brucei | |
0.017 | - |
ATP | pH 6.7, 37°C, at 0.4 mM fructose 6-phosphate | Trypanosoma brucei | |
0.023 | - |
ATP | pH 6.7, 37°C, at 0.75 mM fructose 6-phosphate | Trypanosoma brucei | |
0.026 | - |
ATP | pH 6.7, 37°C, at 2.0 mM fructose 6-phosphate | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | - |
Trypanosoma brucei | 20015 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
4 * 49000, SDS-PAGE | Trypanosoma brucei |
220000 | - |
glycerol density gradient centrifugation | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, ATP-agarose, purification at pH 8.0 in the presence of antipain | Trypanosoma brucei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
140 | 180 | - |
Trypanosoma brucei |
Storage Stability | Organism |
---|---|
-20°C, concentrated enzyme solution in 50 mM Tris-HCl buffer, pH 7.8, 60% glycerol, 1 mM EDTA, 10 mM dithiothreitol, 13% loss of activity per month | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Trypanosoma brucei | ADP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 49000, SDS-PAGE | Trypanosoma brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trypanosoma brucei |