Activating Compound | Comment | Organism | Structure |
---|---|---|---|
citrate | stimulation, by chelating of free Mg2+ | Spinacia oleracea | |
Cl- | 200 mM, 3.1fold activation of cytosolic PFK | Spinacia oleracea | |
HAsO42- | 50 mM, 2.9fold activation of cytosolic PFK | Spinacia oleracea | |
HCO3- | 200 mM, 2.1fold activation of cytosolic PFK | Spinacia oleracea | |
NH4+ | cytosolic isozyme is not activated | Spinacia oleracea | |
NO3- | 50 mM 1.6fold activation of cytosolic PFK | Spinacia oleracea | |
phosphate | 25 mM, 3.2fold activation of cytosolic PFK | Spinacia oleracea | |
SO42- | 50 mM, 2.6fold activation of cytosolic PFK | Spinacia oleracea |
General Stability | Organism |
---|---|
ATP stabilizes | Spinacia oleracea |
glycerol prevents inactivation of dilute cytosolic enzyme solution | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-phosphoglycerate | 1.3 mM, 50% inhibition of cytosolic PFK, no inhibition in the presence of 5 or 25 mM phosphate | Spinacia oleracea | |
diphosphate | - |
Spinacia oleracea | |
K+ | - |
Spinacia oleracea | |
additional information | not inhibited by 3-phosphoglycerate; not inhibited by fructose 2,6-bisphosphate; not inhibited by Na+ | Spinacia oleracea | |
phosphoenolpyruvate | 0.22 mM, 50% inhibition of cytosolic PFK in the presence of 1 mM fructose 6-phosphate, phosphate relives inhibition | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
cytosol | cytosolic and plastidic isoenzyme | Spinacia oleracea | 5829 | - |
plastid | chloroplasts | Spinacia oleracea | 9536 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Spinacia oleracea | |
Mg2+ | MgATP is the active substrate | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cytosolic PFK, ammonium sulfate, DEAE-Sephacel, Blue Sepharose, ATP-agarose | Spinacia oleracea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.5 | - |
cytosolic PFK | Spinacia oleracea |
Storage Stability | Organism |
---|---|
-20°C, 50 mM Tricine-NaOH buffer, pH 7.9, 50% v/v glycerol, at least 2 months | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Spinacia oleracea | ADP + D-fructose 1,6-bisphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-dependence is influenced by Mg2+ and/or phosphate | Spinacia oleracea |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
diphosphate | pH 7.7, 25°C | Spinacia oleracea |